FastQCFastQC Report
Sun 21 May 2023
EGAF00007833651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141714
Sequences flagged as poor quality0
Sequence length247-250
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC11005877.66205173800753No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA137169.67864854566239No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC95506.7389248768646715No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC4650.32812566154367245No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG4250.29989979818507695No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC3210.22651255345272872No Hit
GGAAGTAAAAGTCGTAACAAGGACTACCTAAACCTGTAGGGTTTCACTTG2390.16864953356760798No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2200.15524224847227514No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2040.14395190312883693No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1950.13760108387315298No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1910.13477849753729343No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1760.12419379877782011No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1710.12066556585799569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)