FastQCFastQC Report
Sun 21 May 2023
EGAF00007833660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122941
Sequences flagged as poor quality0
Sequence length235-238
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT3097425.19419884334762No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2619221.304528188317974No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT1778814.46872890248168No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTGAAAGTTG83226.76910062550329No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT50954.1442643219105095No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT50594.114981983227727No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG32472.6411042695276596No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG24742.0123473861445733No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA23151.8830170569622828No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG19021.5470835604070245No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC17081.3892842908386949No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGCAATGGCAACTGTACCAAAATCC12230.9947861169178711No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG10180.8280394660853581No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG9350.7605274074556089No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT8710.7084699164639949No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGTAATGGTAACTGCACCAAAATCC7160.58239318046868No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4780.38880438584361604No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4710.3831105977664083No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAGATTG4510.36684263183152893No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4240.3448808778194418No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT3960.3221057255106108No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG3800.3090913527627073No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT3170.2578472600678374No Hit
GCTGCGTTCTTCATCGATGCCCTTGCCGAGCGCCACGGTGTTTCGTACTG2500.20334957418599164No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG2470.20090937929575975No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTT2080.16918684572274506No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2020.16430645594228127No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG1920.1561724729748416No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGTCAAACT1740.14153130363345018No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1640.13339732066601054No Hit
GCTGCGTTCTTCATCGATGGGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1520.12363654110508293No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCAAGGCCGGACGATCGGGCAAACT1510.12282314280833898No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT1460.11875615132461911No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG1440.11712935473113119No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1440.11712935473113119No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1290.10492838027997169No Hit
GCTGCGTTCTTCATCGATGACAGTCTCGACTATCTCGAGCGAATCGACGA1270.10330158368648376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)