FastQCFastQC Report
Sun 21 May 2023
EGAF00007833661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115286
Sequences flagged as poor quality0
Sequence length247-250
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5472447.46803601478064No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1403012.169734399666915No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC91277.916832919868848No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGTAGGTGAACCTGCCGATGGATC62065.383134118626719No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG49064.2555037038322086No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC29872.590947729993234No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC26862.3298579185677357No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG21751.8866124247523552No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT16341.4173446905955625No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC15951.383515778151727No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT8630.7485731138212792No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG7830.6791804729108478No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC7080.6141248720573182No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT5950.5161077667713339No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG5220.4527869819405652No Hit
GGAAGTAAAAGTCGTAACAAGGCGCGCAAACGGCAGACGCGACCACCGTC5160.44758253387228286No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4830.4189580694967299No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA4350.377322484950471No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT4180.36257654875700435No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC4040.3504328365976788No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCCTTGAT3360.2914490918238121No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG2940.25501795534583555No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT2620.227260898981663No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2530.21945422687923943No Hit
GGAAGTAAAAGTCGTAACAAGGACAGACGGAAGGAAGGAAGAGAAGGAGG2020.1752164182988394No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC1940.16827715420779626No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1870.16220529812813353No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1710.14832676994604724No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT1600.13878528182086292No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG1570.13618305778672174No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC1540.13358083375258054No Hit
GGAAGTAAAAGTCGTAACAAGGGATGGAGATTAGTCAATATTATCGAAAG1520.13184601772981974No Hit
GGAAGTAAAAGTCGTAACAAGGCCTATGAAGAACGCAGCGTCCTTGATAG1510.13097860971843936No Hit
GGAAGTAAAAGTCGTAACAAGGTATCTGTAGGTGAACCTGCAGATGGATC1500.13011120170705895No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1180.1023541453428864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)