FastQCFastQC Report
Sun 21 May 2023
EGAF00007833666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85043
Sequences flagged as poor quality0
Sequence length235-238
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4463652.486389238385286No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1326715.60034335571417No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT975111.465964276895217No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT34434.048540150276918No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT20932.4611079101160587No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT19982.349399715438073No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT10651.2523076561269004No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG4360.5126818197852852No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG4290.5044506896511177No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3540.416260009642181No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3150.3704008560375339No Hit
GCTGCGTTCTTCATCGATGCGAATAACTACGACGAAGAGGGCAAGGTTAC2750.3233658266994344No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT2550.29984831203038464No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT2160.2539891584257376No Hit
TCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2070.24340627682466515No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT1770.2081300048210905No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1520.17873311148477827No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTTGAAAGTTT1490.17520548428442081No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG1120.13169808214667872No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1120.13169808214667872No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG910.10700469174417648No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA870.10230118881036651No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC860.10112531307691403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)