FastQCFastQC Report
Sun 21 May 2023
EGAF00007833671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47831
Sequences flagged as poor quality0
Sequence length247-250
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1342228.061299157450186No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA751015.701114340072339No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC729515.251615061361878No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC20384.260835023311241No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT20284.239928080115407No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC16403.428738684116995No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC13552.832890803035688No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG11632.43147749367565No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC5301.1080679893792729No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA4660.9742635529259268No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGCTGGATC3710.775647592565491No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3550.7421964834521544No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3460.7233802345759026No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC3330.6962012084213167No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT3160.6606594049883966No Hit
GGAAGTAAAAGTCGTAACAAGGTATCGCAGATTTCCAATGCCTGTTCGCC2960.618845518596726No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2890.6042106583596413No Hit
GGAAGTAAAAGTCGTAACAAGGTATCGCAGATTTCCAATGCCTGCTCGCC2560.5352177458133847No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGCATGAAACTCACCGAAAGCTAC2530.5289456628546341No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC1890.3951412264012879No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1800.3763249775250361No Hit
GGAAGTAAAAGTCGTAACAAGGATTACACCACCATTTGCTCTCGCACCAT1680.35123664569003366No Hit
GGAAGTAAAAGTCGTAACAAGCAAGAAGCGCAGCAGTTGAAAGAGAAAAA1500.31360414793753005No Hit
GGAAGTAAAAGTCGTAACAAGGCAGAATTGCTTAAGTGGATGGTTTCTGG1490.3115134536179465No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACGATTAGCACTTGAC1450.3031506763396124No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1410.2947878990612782No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1390.2906065104221112No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1360.2843344274633606No Hit
GGAAGTAAAAGTCGTAACAAGGTACAGGGTCTCTGAGTACTCAACCGGGC1260.2634274842675252No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCAAGGTTGTCCCACAATCGGACTTC1220.2550647069891911No Hit
GGAAGTAAAAGTCGTAACAAGGTCGTCGTCTTCTGAGTATGCGTTTCCAA1200.25088331835002403No Hit
GGAAGTAAAAGTCGTAACAAGGTATCGAAGGTTTGGTACACGTTTCCGAA1190.2487926240304405No Hit
GGAAGTAAAAGTCGTAACAAGTGACCCTTTTTATGGCGCACCAGTCGTTC1130.23624845811293932No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA1080.2257949865150216No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA1070.2237042921954381No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1060.22161359787585458No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1030.215341514917104No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC980.20488804331918628No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC960.20070665468001925No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTTAATAATGCCGTCATCGCCCATC940.19652526604085216No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCCGGCAGGTGTCGTTTTGT930.19443457172126863No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG890.1860717944429345No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG870.18189040580376745No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAGAGGAATTAGCGCAGAATCAGAAA840.17561832284501683No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA720.1505299910100144No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGCGGAAGCCAGTTGTCCATCCCTGT680.1421672137316803No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC640.13380443645334616No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCGAGGTTGTCCCACAATCGGATTTC620.1296230478141791No Hit
GGAAGTAAAAGTCGTAACAAGGCACGGCGCAAGAGCGGAGAAATACGGCC620.1296230478141791No Hit
GGAAGTAAAAGTCGTAACAAGTGGCAAGAAGAATACGATTAGCACTTGAC560.11707888189667788No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTACCGACCTCGCCGTTGATGA530.11080679893792729No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA520.10871610461834376No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA510.10662541029876021No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACAATCAACACTTGAC480.10035332734000962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)