FastQCFastQC Report
Sun 21 May 2023
EGAF00007833675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73363
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3742951.01890598803212No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1010613.775336341207419No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC53497.29114131101509No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC48136.560527786486375No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT21012.8638414459604977No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC19602.671646470291564No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA17982.4508267110123634No Hit
GGAAGTAAAAGTCGTAACAAGTGGCAAGAAGAATACGATTAGCACTTGAC9351.2744844131237818No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA7681.04684922917547No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA7190.9800580674181809No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC3190.4348240938892902No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2570.35031282799231217No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG2300.3135095347791121No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC2150.2930632607717787No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1850.2521707127571119No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC1410.19219497566893393No Hit
GGAAGTAAAAGTCGTAACAAGCACACAATGAACTTACTAATAGTTCAATC1280.17447487152924498No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1200.16357019205866719No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1150.15675476738955604No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1100.1499393427204449No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1090.1485762577866227No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1000.13630849338222265No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC920.12540381391164482No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC900.12267764404400039No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC900.12267764404400039No Hit
GGAAGTAAAAGTCGTAACAAGGGTGCAGGAGCAGATCTTCTCACCATGAC840.11449913444106703No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC830.11313604950724479No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTGGGGCAGAAGGTGGAAAATGCGCG810.11040987963960033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)