FastQCFastQC Report
Sun 21 May 2023
EGAF00007833680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83940
Sequences flagged as poor quality0
Sequence length236-238
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCAAGGCCGGACGATCGGGCAAACT2457229.27329044555635No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1832821.83464379318561No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG78619.365022635215631No Hit
GCTGCGTTCTTCATCGATGCAAGGTAACGACGCTGCGTTCTTCATCGATG74778.90755301405766No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT32043.8170121515368116No Hit
GCTGCGTTCTTCATCGATGCTAAGGTAACGACGCTGCGTTCTTCATCGAT25943.090302597093162No Hit
GCTGCGTTCTTCATCGATGCAAGGTAACGACGCTGCGTTCTTCATCGGCT14241.696449845127472No Hit
GCTGCGTTCTTCATCGATGCAGGTAACGACGCTGCGTTCTTCATCGATGC13321.5868477483917083No Hit
GCTGCGTTCTTCATCGATGAAGGTAACGACGCTGCGTTCTTCATCGATGA11441.3628782463664522No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT10661.2699547295687394No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT8861.0555158446509412No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT7500.8934953538241601No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT7440.8863473909935669No Hit
GCTGCGTTCTTCATCGATGCAAGGTAACGACGCTGCGTTCTTCATCCTTG6240.7433881343817011No Hit
GCTGCGTTCTTCATCGATGTAAGGTAACGACGCTGCGTTCTTCATCGATG5740.6838217774600905No Hit
GCTGCGTTCTTCATCGATGCTAAGGTAACGACGCTGCGTTCTTCATCGGC5480.652847271860853No Hit
GCTGCGTTCTTCATCGATGAAGGTAACGACGCTGCGTTCTTCATCGATGC5240.6242554205384798No Hit
GCTGCGTTCTTCATCGATGAAGGTAACGACGCTGCGTTCTTCATCGGCTC4940.5885156063855135No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA3240.38598999285203717No Hit
GCTGCGTTCTTCATCGATGCTAAGGTAACGACGCTGCGTTCTTCATCCTT2910.34667619728377413No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA2760.3288062902072909No Hit
GCTGCGTTCTTCATCGATGAAGGTAACGACGCTGCGTTCTTCATCCTTGA2550.30378842030021447No Hit
GCTGCGTTCTTCATCGATGCAGGTAACGACGCTGCGTTCTTCATCGGCTC2380.2835358589468668No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2340.2787705503931379No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG1920.22873481057898498No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1620.19299499642601858No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT1220.14534191088873005No Hit
GCTGCGTTCTTCATCGATGCAGGTAACGACGCTGCGTTCTTCATCCTTGA1220.14534191088873005No Hit
GCTGCGTTCTTCATCGATGTAAGGTAACGACGCTGCGTTCTTCATCGGCT1050.12508934953538242No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1030.122706695258518No Hit
GCTGCGTTCTTCATCGATGAAGTCGAGAGAAATTTGTTCTTGTCCGAGGT1030.122706695258518No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1000.11913271384322134No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAACACTGTAATGTCTTC980.11675005956635692No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC910.10841076959733142No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT840.10007147962830593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)