FastQCFastQC Report
Sun 21 May 2023
EGAF00007833681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74827
Sequences flagged as poor quality0
Sequence length247-250
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5062467.6547235623505No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG64568.627901693239073No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC46216.1755783340238155No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA41905.5995830382081335No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC9431.2602402875967231No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC6250.8352599997327168No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT3640.48645542384433427No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA3170.423643871864434No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC3160.4223074558648616No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG3150.4209710398652893No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2910.38889705587555295No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2840.3795421438785465No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC2500.33410399989308676No Hit
GGAAGTAAAAGTCGTAACAAGGGACAGGAGGTGCAGCATGGAAACGCTCG1920.2565918719178906No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC1770.2365456319243054No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1750.2338727999251607No Hit
GGAAGTAAAAGTCGTAACAAGGGCCTCCAATTTGCCCCCTTAAACTCTTA1630.21783580793029253No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1450.19378031993799028No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGGTGGCGTCTTCTGCGAGGTCAGT1410.1884346559397009No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGGGGAAGGATC1190.15903350394910926No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1140.15235142395124754No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA1010.13497801595680703No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGCGCAGCCAAGGGCTCTGCACCCAC970.12963235195851763No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC940.1256231039598006No Hit
GGAAGTAAAAGTCGTAACAAGCAGCTTGGTCCAGGGCGCCGCCCGAAGTG920.12295027196065592No Hit
GGAAGTAAAAGTCGTAACAAGGAGACACAAAACTGCCGACTTTCCCGACG850.11359535996364949No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC810.1082496959653601No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC770.10290403196707072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)