FastQCFastQC Report
Sun 21 May 2023
EGAF00007833685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121793
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5034241.33406681829005No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA3317827.241302866338792No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAGTGCTTG47863.929618286765249No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC45083.7013621472498417No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC39383.233354954718252No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC33132.7201891734336128No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG24321.9968306881347861No Hit
GGAAGTAAAAGTCGTAACAAGGGCGAAGGCAGAGCGGGAGAAGGAAGGAA18151.4902334288505907No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC12811.0517845853209955No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC6740.5533979785373544No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA6570.5394398692864122No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC6400.52548176003547No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC6380.5238396295353591No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG5950.48853382378297605No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGGGTGCAGGAAAAATGTTGCAGA5360.4400909740297061No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT5250.4310592562790965No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC4700.3859006675260483No Hit
GGAAGTAAAAGTCGTAACAAGGGCGAAGGCAGAGCGAGAGAAGGAAGGAA3960.3251418390219471No Hit
GGAAGTAAAAGTCGTAACAAGGGGAAGTAGACCTGGCCCTCTTCCAGGGC3790.3111837297710049No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT2990.24549850976657117No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG2750.225792943765241No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG2390.19623459476324584No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA2240.18391861601241452No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA2020.16585518051119522No Hit
GGAAGTAAAAGTCGTAACAAGGTTCTGCATCGATGAAGAACGCAGCCAGT2000.16421305001108438No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGCAGAAAAGCATGATGATAGCCC1920.157644528010641No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT1850.15189707126025304No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1830.15025494076014223No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1810.14861281026003137No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1760.14450748400975424No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACGATTAGCACTTGAC1550.1272651137585904No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAACCGAAGTCATGTTGCGCCCGCTG1470.12069659175814702No Hit
GGAAGTAAAAGTCGTAACAAGGTCTGCATCGATGAAGAACGCAGCCAGTG1470.12069659175814702No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC1330.10920167825737111No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1270.10427528675703858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)