FastQCFastQC Report
Sun 21 May 2023
EGAF00007833711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82548
Sequences flagged as poor quality0
Sequence length247-250
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5991672.58322430585841No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA897210.868827833502932No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC13991.6947715268692154No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC11271.3652662693220914No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC9091.1011774967291756No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC8391.016378349566313No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTCCATTGCG7140.8649513010612007No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA6880.8334544749721374No Hit
GGAAGTAAAAGTCGTAACAAGGATATATTCTGCTGTATAGAAGCAAGGTT3320.4021902408295779No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCTAATCGACGCAATCG2840.3440422542036149No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCTGCCCTTGCTGCAGGATTCCA2840.3440422542036149No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2100.25439744148858845No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1970.2386490284440568No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC1960.23743761205601588No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1940.23501477927993408No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAGCTCTCCGGCA1860.22532344817560693No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTCAC1830.22168919901148423No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1770.21442070068323885No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC1330.16111837960943934No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG1270.15384988128119395No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1220.1477927993409895No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG1170.141735717400785No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA1080.13083296990841692No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1000.12114163880408975No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC950.11508455686388526No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT940.11387314047584436No Hit
GGAAGTAAAAGTCGTAACAAGGTATCACAGATTTCCAATGCCTGTTCGCC920.11145030769976257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)