FastQCFastQC Report
Sun 21 May 2023
EGAF00007833715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102191
Sequences flagged as poor quality0
Sequence length247-250
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5776756.528461410496035No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2772627.131547787965673No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC26342.5775263966494113No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA19791.9365697566321887No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC13411.3122486324627414No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT9890.9677955984382187No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC6170.6037713692986663No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG5020.49123699738724547No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC3440.3366245559785108No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3210.31411768159622666No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA2820.2759538511219187No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC2440.23876858040336232No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC2250.2201759450440841No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACATGCAGAAGGATC1850.18103355481402472No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1700.16635515847775245No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1580.15461244140873462No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1570.15363388165298314No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC1560.15265532189723166No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1470.1438482840954683No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATC1460.1428697243397168No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA1440.14091260482821383No Hit
GGAAGTAAAAGTCGTAACAAGGTTTACGTAGGTGAACCTGCGGAAGGATC1430.13993404507246235No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGAGGAAGTG1240.12134140971318413No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG1240.12134140971318413No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA1200.11742717069017819No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1190.11644861093442671No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1170.11449149142292374No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC1140.11155581215566929No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1110.10862013288841484No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGGGGCAGGCCATTGACCCCTCTGA1100.10764157313266334No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1080.10568445362116037No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1070.10470589386540889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)