FastQCFastQC Report
Sun 21 May 2023
EGAF00007833733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189749
Sequences flagged as poor quality0
Sequence length247-250
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC15045279.2900094335148No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC61023.215827224385899No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG28291.49091694817891No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG22481.1847229761421667No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC19921.0498079041259771No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC19821.0445377841253445No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC18320.9654859841158584No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT14710.7752346520930282No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC14150.7457219800894866No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC12690.6687782280802533No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC9100.4795809200575497No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC5740.3025048880363006No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA5090.2682491080321899No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTAAGCAGAAAACAAAACAAACAACG4930.259816916031178No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG4000.21080480002529656No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATC3830.2018455960242215No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC3480.18340017602200803No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA3400.17918408002150207No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA3060.16126567201935188No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC3040.1602116480192254No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC2760.14545531201745465No Hit
GGAAGTAAAAGTCGTAACAAGGGAAAAGTATAGAGAAACGTCCCGTCTGG2670.14071220401688544No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA2610.137550132016506No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA2460.1296449520155574No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2390.1259558680151147No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCGGCAATCCGATAGATTCAGACTTG2250.11857770001422932No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2140.11278056801353367No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2120.11172654401340719No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1950.10276734001233208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)