FastQCFastQC Report
Sun 21 May 2023
EGAF00007833743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177133
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC9008850.85895908723953No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG4321324.395792991706795No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA96365.439980127926473No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG82344.6484844721198195No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC29991.693078082570724No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC17270.9749736074023473No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT15720.8874687381797858No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC14790.8349658166462489No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC9680.5464820219834813No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC9070.5120446218378281No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA7890.44542801172000696No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC7670.43300796576583694No Hit
GGAAGTAAAAGTCGTAACAAGCCAAAACCTGTTACATCAGTCATCGCTTT6330.35735859495407407No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT6230.3517131195203604No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC5800.32743757515539174No Hit
GGAAGTAAAAGTCGTAACAAGGTTATCGTAGGTGAACCTGCGATAGGATC4950.27945103396882565No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA4600.25969186995082794No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG4000.225819017348546No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT3860.21791535174134688No Hit
GGAAGTAAAAGTCGTAACAAGGGCACAGCATATCGGGGATTAGTCTATTC3770.21283442385100462No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG3620.20436621070043415No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC2510.1417014333862126No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACGACATAAACTACTTGAGTACTAA2210.12476500708507167No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2150.12137772182484348No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2130.12024862673810076No Hit
GGAAGTAAAAGTCGTAACAAGCAGCTTGGTCCAGGGCGCCGCCCGAAGTG1970.11121586604415891No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1910.10782858078393072No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1820.10274765289358843No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)