FastQCFastQC Report
Sun 21 May 2023
EGAF00007833765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116302
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4313037.08448693917559No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG2068817.788172172447595No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1245110.705748826331448No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC63195.433268559440078No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG41733.588072432116387No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG30922.658595724923045No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA22771.9578339151519322No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC22051.8959261233684719No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG15661.3464944712902616No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC15291.3146807449570945No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG11530.9913844989768017No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC10610.9122800983646024No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC10320.887345015562931No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA9630.8280167151037816No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC9340.80308163230211No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC7380.634554865780468No Hit
GGAAGTAAAAGTCGTAACAAGGCAGAAAAGGCAGCCGTCATCGGCGATGG5710.49096318206049766No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGGAAGAGCCGCCACCTTTGAAGT5460.46946742102457395No Hit
GGAAGTAAAAGTCGTAACAAGGTGACTGTTGAGTGAATTTCTTATACAAA3970.34135268525046863No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC3340.2871833674399409No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGGTCAAAGTTGCCGATAAGACTAT2970.25536964110677374No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGGAAGGAAGCGATGGGATGCTGTC2960.2545098106653368No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC2800.24075252360234561No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTTAATAATGCCGTCCTCGCCCATC2680.2304345583051022No Hit
GGAAGTAAAAGTCGTAACAAGGCACTGAAGGGGAAGGGAGGGAAGACGAT2580.22183625389073275No Hit
GGAAGTAAAAGTCGTAACAAGGCATTCACGGTGTGTATTTACGAGTTCAT2300.1977610015304982No Hit
GGAAGTAAAAGTCGTAACAACAGCGAAGAAACACCACAGACCAAGATTCA2040.17540541005313753No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1920.16508744475589415No Hit
GGAAGTAAAAGTCGTAACAAGGCCCTCCTGGCGGCTCACGGCCCCGAGCT1890.16250795343158328No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGACAAGCTGATGATTGTGGGAGCAT1860.15992846210727243No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1760.15133015769290295No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGGGGAAGGGATCCATGGGCAGCA1720.14789083592715516No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCTATCGCAGCGGGCTTCCA1630.14015236195422262No Hit
GGAAGTAAAAGTCGTAACAAGGCTACGGAAAAGAAGGTTTTGGTGGGGAA1510.12983439665697924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)