FastQCFastQC Report
Sun 21 May 2023
EGAF00007833777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62542
Sequences flagged as poor quality0
Sequence length247-250
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2996047.90380864059352No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG753712.051101659684692No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG701111.21006683508682No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC25194.027693390041892No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA21573.4488823510600874No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC18712.9915896517540213No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC17552.8061142911963164No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC14322.2896613475744303No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC7481.195996290492789No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT4500.7195164849221323No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4000.6395702088196732No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC2330.3725496466374596No Hit
GGAAGTAAAAGTCGTAACAAGGTTGCTGTAGGTGAACCTTCAGCAGGATC2190.35016468932877104No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1650.26382271113811523No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA1590.2542291580058201No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1570.2510313069617217No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1510.24143775382942664No Hit
GGAAGTAAAAGTCGTAACAAGGTCCTGTGAGAGAAGGACAGAACTGAGGT1480.2366409772632791No Hit
GGAAGTAAAAGTCGTAACAAGGTAAATGGAATCTTGGCAAAAAGTTCGAC1430.22864634965303315No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1350.2158549454766397No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1310.209459243388443No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA1150.18387643503565604No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1100.17588180742541012No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1030.16468932877106585No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG1020.16309040324901666No Hit
GGAAGTAAAAGTCGTAACAAGGGATATACGCAGTGCCCGGTAACTTCATA990.15829362668286912No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC970.15509577563877075No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC960.15349685011672157No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC950.15189792459467238No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTTCCAGGTT950.15189792459467238No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA870.13910652041827892No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG870.13910652041827892No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC800.12791404176393462No Hit
GGAAGTAAAAGTCGTAACAAGGTTTAATCAATGAAAACTACGTCGGCATC790.12631511624188546No Hit
GGAAGTAAAAGTCGTAACAAGGCCAAGTCGATGAGTTGCGGCACCACTTG760.1215183396757379No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA690.11032586102139362No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA640.10233123341114772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)