FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007833820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162692047
Sequences flagged as poor quality0
Sequence length89
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39232682.4114688285900048No Hit
GGGGGGGGGGGGGGGGGGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2103320.12928228753554255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCGGCCTTAGCGCCAT1850610.11374926028191164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1848230.11360297163142831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1805880.11099989417429851No Hit
GGGGGGGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1688360.10377643106303776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTTTCCGTCTCAGGTCG1651110.10148682928551511Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)