FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007833846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10717660
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA2302422.1482487781847905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGCCCGTCCCCGCCCCTT1223431.1415085009227761Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTCCCGCGGGGCCCGAAG1151751.0746282304159676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGACCGCGGTTCTATTTTG525260.49008832151794324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC515380.4808698913755428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGAAG444610.41483868680290287Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA387470.361524810453028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT380310.35484424771825196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCCGAAGCGTTTACTT367080.34250013529072576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA354740.33098642800760614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATGACCCGCCGGGCAGCT324840.3030885473134994Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGAA316970.2957455265421743Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGTCCCGCGGGGCCCGAA314270.2932263199243118Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT308890.28820656747834883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGCCTGGATACCGCAGCT292710.2731099885609359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCACCGCCCGTCCCCGCCCCT276590.25806939201280876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGCCCGAAGCGTTTAC265150.24739542026897665Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG247310.23074999580132233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGGGGGATGCGTGCATT244510.2281374852346501Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGACCGCCCGTCCCCGCCCCTTGC239600.22355626134809278Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAATGTTTTCATTAATCA234800.21907767180522614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGTCCCGCGGGGCCCGAAGCG226840.21165067747997232Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA226620.2114454087925909No Hit
GTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGAAGCGT222040.20717208793710568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG195160.18209198649705255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG187550.17499155599263272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC187080.17455302743322704Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGGGACGGCCGGGGGCA179610.16758322245714083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCGAGCCACCGCCCGTCC171180.1597176995724813Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGACCGCCCGTCCCCGCCCCTTG169490.158140862837597Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA167330.156125497543307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGTGTCCCGCGGGGCCCGAAGC161080.15029400074269944Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAACTTCTTAGAGGGACAA156570.146085992651381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCCTGGATACCGCAGC153650.1433615173461371Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGAAGC149350.1393494475473191Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCGGCGCCCCCTCGATG148590.1386403375363652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA143220.13362991548528316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCC142940.13336866442861595No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC141040.13159588940123124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATACCGTCGTAGTTCCGA141000.13155856782170733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA137910.12867547580348696No Hit
GCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCA132840.12394496559883408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC131040.12226549452025907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAACGAAAGTCGGAGGTT129570.12089392647275617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCGAGCCACCGCCCGTC129130.12048338909799342Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT128910.12027812041061202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGCCCGAAGCGTTTACT127580.11903717789144273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT126990.11848668459346537No Hit
GGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAA125780.11735770681286772No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA124730.11637801535036565No Hit
GCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG123210.11495979532845789No Hit
GGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGC120410.1123472847617857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATTACCCACTCCCGACCC119910.11188076501773708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG118390.11046254499582932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117680.1098000869592803No Hit
TGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAA117320.1094641927435653No Hit
GTGGTATCAACGCAGAGTACATGGGGTGTCCCGCGGGGCCCGAAGCGTTT117120.10927758484594585No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC116570.1087644131274924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCGCCGGGCAGCTTC115700.1079526687728478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAA114400.10673971743832143No Hit
GTGGTATCAACGCAGAGTACATGGGACCGCCCGTCCCCGCCCCTTGCCTC113910.1062825280891538No Hit
GGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGT113120.10554542689355699No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC109220.10190657288997784Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)