FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007833864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14749676
Sequences flagged as poor quality0
Sequence length89
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4596173.116115906546015No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4588753.1110852875683506No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4316892.9267693744594796No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180332.834184289878639No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3979632.698113504323756No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3911052.651617567735047No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3894682.640519018858448No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3747032.5404151250508824No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3623372.456575995296439No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160452.142725033417683No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2832001.9200421758416932No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2303151.5614919270091085No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2046221.3872982701450527No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1957791.3273444108196002No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1908221.2937368929324278No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1808611.2262032060907642No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1488161.0089441964691293No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1394640.9455394138827184No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000510.6783267646014733No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA988790.670380827348343No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA856220.5805008869347367No Hit
CTCCTCTGCAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA627430.4253856152501248No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT477990.32406813546277224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG457030.3098576538223619No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT454990.30847457259400135No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT422580.2865012085689204No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT406720.2757484299994115No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT375240.2544055882990243No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT372190.25233774626642647No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC368930.25012752822502676No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT364580.2471783108998462No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT362600.24583590853114332No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC344610.23363903044378737No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT331640.22484561694778923No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC323240.21915057659571643No Hit
TGTCTTTCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316710.2147233607029741No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT313780.21273687638969155No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC311250.21102158447412678No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG293570.19903488049500206No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC279930.18978721973282667No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT276580.18751598340194048No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC269410.18265485967284978No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC268610.18211247487741428No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC268130.18178704400015294No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG263060.17834968035908041No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT259290.1757936920105906No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG252970.17150885212665012No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT248600.16854607518158365No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT239560.16241712699316244No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236490.1603357253406787No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG235220.1594746894779248No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC231970.15727125124646804No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG218490.14813206744337978No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG218250.14796935200474912No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC216060.14648457362724443No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC214430.14537946460654458No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT210190.14250482519073637No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT210020.14238956842170636No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT205690.13945391071641167No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT202600.137358949444042No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT202210.1370945368562672No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC198330.134463970598405No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG198250.13440973211886145No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188130.1275485644566023No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA187640.12721635376939805No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT185040.12545360318423265No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA184960.1253993647046891No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG184290.12494511743851187No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT184190.12487731933908243No Hit
TGATGGCCTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180550.12240946851985088No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT179840.12192810201390188No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA175250.11881616925009066No Hit
CTCCTCTGCAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175170.11876193077054709No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT174740.1184703989430005No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC174490.1183009036944269No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG171810.11648391462971797No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT170620.11567711724650766No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG167220.11337198186590675No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC166760.11306011060853133No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG166350.11278213840087063No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC164300.11139227736256715No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT162760.11034818663135379No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA156630.10619216313632923No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA152310.1032632852409775No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA151810.1029242947438303No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC147700.10013779285728039No Hit

[OK]Adapter Content

Adapter graph