FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007833958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89624541
Sequences flagged as poor quality0
Sequence length89
%GC19

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43153734.814945718940977No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40827354.555376188760621No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37617764.197261105080583No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26657842.974390686140306No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26222162.9257790006422457No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19331382.1569293169378683No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18672402.0834025805498966No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18097662.0192750554783876No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16592041.8512831212156502No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11793391.3158661532224751No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10164651.1341369101125998No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9620481.0734202811705336No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9320221.0399182964853342No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9051151.0098963854107772No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8004360.8930991345328062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7365570.8218251293471059No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6269620.6995427736695466No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5301350.591506516055686No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4734930.528307308151235No Hit
GGTTGCCAGATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4512240.5034603189766964No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4032060.4498834755538664No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3645710.406775862874433No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3514610.3921481728983136No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3392810.3785581451401798No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2888840.32232689481779325No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2657750.2965426623495902No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630970.293554641468122No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2486500.2774351725829201No Hit
GTCAACTCTTTAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2474780.27612749503509315No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2389980.2666658008323859No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2211630.24676611732940423No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2143500.23916440475829048No Hit
TGTCTTTCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2101880.23452058739134854No Hit
TTCCGCCTCTCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080300.23211276473929168No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1874960.20920162927250024No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1795970.20038819501457752No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1760840.1964685096685739No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1728170.19282330271571488No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1658530.1850531095049067No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1599500.17846674383526273No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1586150.17697719645783178No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1576570.17590829279672404No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1549520.17289014623795954No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1459320.16282593848932517No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1373930.1532984141028962No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1353060.1509698108244705No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1251410.13962805120530547No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1251180.13960238859131227No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1226700.13687099384977602No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1224460.13662106230479887No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1217520.13584672082169993No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1132440.1263537851758705No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1123910.12540203692647084No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1119920.12495684636198025No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1114990.12440677380986531No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1093750.12203688719588533No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1091200.12175236691030863No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1077430.1202159573681945No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1067410.11909796001075197No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1063700.11868401088938352No Hit
TTCCGCCTCTCTTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1036230.11561900216593578No Hit
TTCCGCCTCTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1013110.1130393515767071No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT966060.10778967336636067No Hit
GGTTGCCAGATGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA948500.10583038857627175No Hit
TTCCGCCTCTCTTTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA944810.10541867098655491No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT930720.10384655693801545No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC922920.1029762595938985No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT918740.10250986947871789No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT917510.1023726302821456No Hit
GTCAACTCTTTAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913840.10196314422408033No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA904310.10089981939210155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)