FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007833960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129485839
Sequences flagged as poor quality0
Sequence length89
%GC19

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70708805.460736135014733No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45768653.534645205488455No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45300143.4984628705228533No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44633473.446976931585546No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32777902.5313887798958463No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32347052.4981148710786822No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31128742.404026590120021No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26389992.038059930244573No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25282871.9525586886763733No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23191551.7910491354965852No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21008661.6224677665331417No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20392451.574878778829243No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18477521.4269915646914872No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18296481.4130101130209305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16969911.3105610722420387No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13694691.057620671554671No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12015180.9279145961281527No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11973970.9247320087256801No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11361920.877464291674397No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8717560.6732442765420857No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470860.6541920000997176No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6286370.4854870655006529No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5540170.4278591421877415No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4595970.35493997146668677No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4083760.3153827500781765No Hit
GTCAACTCTTTAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3947280.30484260135967456No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3901580.3013132578922395No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3372470.2604508744774786No Hit
GGTTGCCAGATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3164240.2443695792865813No Hit
TGTCTTTCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3152150.24343588645241737No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3119560.24091900891185483No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3021500.23334598001871076No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2760820.21321404883510078No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2689130.20767753607404127No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2658680.20532592757112228No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2498200.19293229431829995No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2383930.18410739107926696No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2383290.18405796482501843No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2285190.17648184679098383No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2284470.1764262422549542No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2226640.17196011681246473No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2209760.17065649935665939No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2120490.16376230917421017No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2114670.16331283917463746No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1930130.14906108767615894No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1877810.1450204913913405No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1823290.14080999235754268No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1787000.13800736928460572No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1769900.13668676155390244No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1662240.12837233884703023No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1632650.1260871468732577No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1591540.12291228232301141No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1584370.12235855381838318No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1564690.12083869650024047No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1559160.12041162277212415No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1534320.11849326627910253No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA1522360.11756961315283287No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1510150.11662665289599737No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC1506790.11636716506119252No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1483730.11458627533779968No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1417580.1094776085900791No Hit
GTCAACTCTTTAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1399160.10805505920998822No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1391930.1074966969940242No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1385860.10702791986388566No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1377600.10639001226999038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)