FastQCFastQC Report
Thu 12 Jan 2023
EGAF00007834156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26929503
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCATTCAATGGTAA1455120.5403441719663374No Hit
ATTACTCAGGACCGAT967120.3591302817582634No Hit
GGCACGTAGTGAAACT955710.35489329305483286No Hit
CAGTATGTCGCTACCT920320.34175157261535793No Hit
AAAGGGCGTAGTACAA916260.34024393246321705No Hit
TTTGTGTAGTTGAATG823090.30564619035115503No Hit
CAATCCCTCGCCCTTA818160.3038154844521267No Hit
CTACTTAAGGATGTCG776530.2883566027935978No Hit
TGCCTCAAGTCTCCAA752270.2793478958746472No Hit
GGAACCCCAATTGGCT748980.2781261874754985No Hit
ACCGAATGTCGAGGTA694790.25800327618374536No Hit
AACATCGGTTTGAAGA675650.2508958297522238No Hit
TCCAGAAAGTCTCTAG668070.24808107301497545No Hit
AGGCCCATCACCACAA633260.2351547297400921No Hit
CCTGGGACAAGGAAGA632240.23477596300236214No Hit
CCCTCTCGTAACCCAT628460.23337229803312742No Hit
TTACCGCCAGATGGCA598860.2223806358401787No Hit
TAGGTCAAGTTGTCCA597140.22174193114518304No Hit
TCCCACAAGCGTGTTT585110.21727471167960286No Hit
AGGACGAGTCCCGAGT559060.20760130626993004No Hit
ACCGGGTTCATTGCCC550230.20432237460899297No Hit
GAACTTGTCGTGGCGT546680.203004117825717No Hit
TGCTCGTTCTAGCTGA545950.2027330396702828No Hit
TGACAACCACCTGGTG543770.2019235186033697No Hit
CGCACAGGTCTAACCA531580.19739688474755737No Hit
ATGTCTTCAGGTAACG529700.19669876566232952No Hit
CGCAATAAGGTCACTT525530.19515027811690397No Hit
GTAGACTGTGGTGGTA522010.19384316153179657No Hit
TTCTGTATCCACGAGC511900.19008891474900225No Hit
AGGACGAAGGATATCA503950.187136762234342No Hit
CACGTTCTCGCTTACC500980.18603388261565762No Hit
CGCGCAACACATGATC490550.182160807052399No Hit
AACCGATAGGCGACTA488440.18137727978121246No Hit
ACTGTCCGTATCCTTT475690.17664269555958756No Hit
ATTCGTTAGGGAAATG474180.1760819722517716No Hit
GGAGGATTCACGATTG474120.17605969185543455No Hit
TACTAGGAGCCTGTAT462100.17159618578924388No Hit
GCTGCGAGTTTCGATG458600.17029649600291547No Hit
GGCGTTGGTCAGCTTA454890.16891882482940734No Hit
ACCGCAGGTAGTGATA445630.16548021699472135No Hit
GGGTTATCATGATCGT438390.16279171583671634No Hit
GGAGTAGGTGTCTACA434880.16148831265099842No Hit
AGTGTACTCGATGCAT434200.16123580149251177No Hit
ATTGTCTGTTTCCGGG428210.15901147525819545No Hit
ACAAGCTGTATCCTTT421710.15659776565501413No Hit
CTCACCAAGTCTTAGC419270.15569169620397375No Hit
ATTACTCGTCCCGAGT416040.15449226820116213No Hit
GGCATTAAGGTCGGTA406810.15106480056464464No Hit
ACATGGTGTGATCAGG404220.15010303012276163No Hit
CCCAGAGTCGTGAGTT394140.14635992353813587No Hit
CGCTGGACATTACCTT392650.1458066270290989No Hit
AGTGTACTCGCCCTTA388620.1443101270751265No Hit
GAGCATTTCTTAGTGG387500.1438942263435014No Hit
CCTTGCATCAGTCAAC369080.1370541446680245No Hit
CCTAAAGCACAGATGG369010.13702815087229794No Hit
AAACTCGTCCAGGTCG364890.13549823032381994No Hit
TTCGCGTAGATATGAC364500.13535340774762905No Hit
TTAGGTGGTACCTATG362200.13449932588804184No Hit
TGCCTCATCCAGAGAG358610.13316621550720784No Hit
CGGACTGTCGTCGCAG358450.13310680111697568No Hit
TGTGTCCAGTGATAAC356170.13226014605616745No Hit
TCAAGCAGTGATAAGT350850.13028461758094828No Hit
GCGGAAAAGTCCGATT350570.13018064239804203No Hit
GCGGGTTTCGCCACTT349930.12994298483711342No Hit
GAACCTATCAAAGTAG349670.12984643645298616No Hit
TAACAGCCATGTGAGG349340.12972389427313233No Hit
GCAAAGGAGGCAATTA348060.12924857915127508No Hit
ATAGTCGAGTGTCACT345140.12816426652953825No Hit
TCGCCTATCTGTTTAC339030.1258953795025478No Hit
TCAAGGTGTTGGAGGT337670.1253903571855745No Hit
TCACCACCAGGCAAGT332630.1235188038932616No Hit
GTGCTGGGTTCTACGA326720.12132418485406135No Hit
TCAGTCCTCCATCGAA318750.11836460554062213No Hit
GCCTACTGTATGCTAC317070.11774075444318449No Hit
TGCATTTTCACGTCAA315230.1170574889555147No Hit
TGCACCTGTCACAGTT310840.11542730662351994No Hit
CAGGATTGTTACTTTC308310.11448781657797398No Hit
GATCGTACACGTTGTA303230.11260140968810303No Hit
GACCCAGTCCGAATTG298510.11084868517625444No Hit
GAAGAGCAGTGAGCTG298090.11069272240189505No Hit
GCTTGCTTCGAGCGCT296660.11016170628919517No Hit
GTGCACGTCCCAATGA295050.10956384898748409No Hit
AGGCGAAGTTCCTATT291180.10812676342374383No Hit
GGTACCGGTATCCTTT288520.10713899918613425No Hit
TAATTCCCAAGGATGC284660.10570562702178352No Hit
AGGCGAATCTGTAGAC282900.1050520687292298No Hit
CGCTGGAAGCTACGTT281610.10457304020798305No Hit
CAGCTAAAGAGGTCCA281570.104558186610425No Hit
CAGTGTAGTTTGGATC279840.10391576851603983No Hit
AGGACGATCGGTGATT275630.10235242737305623No Hit
TTCATCAAGCAATGTA274580.10196252043715771No Hit
CGCTATCAGGCCTCTG274260.10184369165669341No Hit
TGGGTGCCATCAGCTA273600.10159860729698575No Hit
CTCAGAACATGGTTTG272320.10112329217512853No Hit
CTGCTCAAGACCATAA270760.100544001870365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)