FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007834298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920461
Sequences flagged as poor quality0
Sequence length89
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4293673.3231554199188404No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3915843.0307277735678317No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3429582.6543789730103287No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3266422.528098649111669No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000512.32229329897749No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2715682.101844508489287No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2447801.8945144449567242No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2222671.7202714361352895No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1767101.3676756580125122No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1395301.0799150277997045No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1039040.8041818322117145No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002220.77568439701958No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894080.6919876930087866No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA868730.6723676500397315No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827870.6407433914316215No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788550.6103110407593042No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT706090.54648978856095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG681590.527527616855157No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT659370.5103300880672911No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT643530.49807046358485196No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT564790.43712836562100993No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT556550.43075088419832697No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA549150.4250235343769854No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT481050.3723164366968021No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433050.33516605947728956No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT386310.2989908796597892No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC371440.28748200238366106No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT361990.28016802186856954No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC360100.27870522576555123No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT354280.27420074252768534No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT353330.2734654746452158No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT342460.2650524621373804No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT340290.2633729555005816No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC336320.2603003097180511No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT332780.25756046939811206No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT318480.2464927528514656No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC313720.24280867377719728No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG307730.23817261628667893No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC303560.23494517726573377No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG299690.2319499281024106No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298640.23113726360073375No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT275430.2131735082827153No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA267650.207152051308386No Hit
CTCCTCTGCAATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263790.2041645418069835No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG255170.1974929532313127No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC254950.19732268066905662No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG241150.18664194721844676No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT239300.1852101097631114No Hit
AGTAAGTTCAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA232410.1798774826997272No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA225150.17425848814527592No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG216310.16741662700734905No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA211930.16402665508606853No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT209630.1622465328443002No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG206080.15949895286244045No Hit
CTCCTCTGCAATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205690.1591971060475319No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT203700.15765691332530626No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA203630.15760273569186115No Hit
AAGTATCGTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200140.15490159368152576No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA195290.15114785764997085No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC188740.14607837909189153No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA180760.13990212887914757No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT179480.13891145215329392No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC179230.13871796060527564No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC179120.13863282432414756No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC176870.13689140039198291No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT175220.13561435617506218No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT169350.13107117462759263No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG163670.12667504665661697No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA161480.12498006069597671No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG159100.12313802115884256No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT156600.12120310567865962No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC156100.12081612258262303No Hit
GTCAACTCTTTAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155280.12018147030512301No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT150950.11683019669344616No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC149530.11573116470070224No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG148940.11527452464737907No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT145080.1122870151459766No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG143210.11083969836679976No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC138370.10709370199716557No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC137320.10628103749548874No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT136350.10553029028917775No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT135130.10458605153484848No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG134420.10403653553847654No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA132690.10269757402618994No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC131760.10197778546756188No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG131250.10158306270960456No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG130850.1012734762327753No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT130610.10108772434667772No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG129910.10054594801222651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)