FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007834550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114433144
Sequences flagged as poor quality0
Sequence length16
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTCGCAGTTCCCA14719521.28629866186321No Hit
TTGCTCCGTTAAAGGT13922471.2166466386696497No Hit
GTTGGTGAGTTTCTGG12425071.0857929412478609No Hit
GTAGGCGGTCTAGCGC12257211.071124114181465No Hit
GGCGTAATCCTAATCG12199461.0660774993650441No Hit
ACACTTGTCAGCATGG11947291.0440410516030216No Hit
CGAAGAAAGAACAACC11807281.0318059599935487No Hit
GGACAAGCAAGGTCAC11312800.988594702947251No Hit
TTGTTGTAGCTTGTTG11213350.9799040389906616No Hit
CTACTAGTCAGGTCGA11159160.9751685228538334No Hit
TTAGGCCTCAGAAAGG10918700.9541553800182228No Hit
TTTCAAGGTCCAGCAC9399740.8214176130649701No Hit
TGACGGATCCAGGTGA8925730.7799951734263283No Hit
ACCTGGTTCCGTCCAG8273450.7229942052452915No Hit
GCGATAAGTAGCAGGT6948260.6071894695124344No Hit
CAACCGTTCAGCTACC6390860.5584798054661506No Hit
TAGAGGCGTTATTGTG6315880.5519275079954108No Hit
GCACAGCAGCACAGCA6164300.5386813456772629No Hit
TTACCGGGTTACTTCT5528990.4831633394604626No Hit
TAATGCAAGGACGAAA5263040.45992269512406303No Hit
TTTGCTCTCCTAACCA4919180.4298737086171468No Hit
ATGCTTAGTTCACCTC4778910.41761589631759133No Hit
TAGGATATCAAGCTTT4049350.35386163994585346No Hit
CCTTACCGTAGTCATC3690970.32254379028509433No Hit
GGTCATGAGAAGCAAG3533910.30881874572982104No Hit
TGATGGCCAGGTTGCG3438760.3005038470322899No Hit
CGTAGTTGTTACGCCA3434330.3001167214281904No Hit
GTGGCGTAGTGAGGCC3165710.2766427530820965No Hit
GATTACTTCATAGCCC3137550.27418192757161336No Hit
TGTCAATTCATTAAGG2892430.25276156005990713No Hit
ATTTACGGTTAACGGA2523010.22047895494333355No Hit
GTGTTGCTCCAACCTA2428570.21222610120718174No Hit
TCACCTGCAGAACCGT2391100.2089517002172028No Hit
TCACCAGGTTATGGGA2381920.20814948508274841No Hit
GGCCTTAGTTATCCGG2186860.1911037242846356No Hit
GGTTTACGTATTGCGT1972410.17236352432997906No Hit
GAAGCTAGTGCTCCTT1915800.1674165310008436No Hit
CTTTGTGAGGTTGCGA1896370.16571859635351802No Hit
TCCTTGCGTACGTAAG1850670.16172499813515567No Hit
GACGAGGAGGCATGGC1721830.15046602232653855No Hit
TAGTTTGGTGGTTAAG1534300.13407828766812524No Hit
GCCTTCCTCCCACCCT1522670.13306197372327724No Hit
GTATCCGGTGTTAACC1513210.13223529015334928No Hit
CTGAGTCTCGGTTACC1489840.13019304966400294No Hit
ATTTGTGGTCGATTAC1485710.12983214024076803No Hit
TGTTTGTAGCAATACA1474670.12886738478495355No Hit
ACCTGGAAGCGCTACT1465880.12809925068562306No Hit
GTAATAGTCCGTCCCT1439630.12580533485997727No Hit
CTAGTAAAGCAGGTTG1422980.12435033682199625No Hit
GCTTAGCTCCAAATAG1362880.11909836192213683No Hit
CGGAACGGTCGAGGCA1333520.1165326716881955No Hit
CCCTTTAGTCTATCTT1331280.11633692420440708No Hit
CCTCCAACAGAGAGCA1309600.11444236820059754No Hit
TGTTGACGTAACCGGG1296800.1133238111503779No Hit
GGTGAGGAGTTAGGAC1295670.11322506353578819No Hit
CTGTGCAGTACTTATG1286350.11241061418359702No Hit
TTCAGGACAAATATGC1272130.11116796721061863No Hit
TATTGTGGTAGCTAAG1261020.1101970946459358No Hit
TCTAACCCAATGACCA1248170.10907416823223873No Hit
TCTCAATAGCTTGGTC1243420.10865907870188378No Hit
TTGAGCGAGGAGCAAA1190260.10401357145269031No Hit
CTACAACGTCACCATG1168410.10210415961305755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)