FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007834571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115643086
Sequences flagged as poor quality0
Sequence length16
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTCGCAGTTCCCA14957441.293414117295348No Hit
TTGCTCCGTTAAAGGT14161141.2245556989027429No Hit
GTTGGTGAGTTTCTGG12617381.0910622015050688No Hit
GTAGGCGGTCTAGCGC12457881.077269764316044No Hit
GGCGTAATCCTAATCG12353031.0682030744146693No Hit
ACACTTGTCAGCATGG12103731.0466453653787828No Hit
CGAAGAAAGAACAACC12023011.0396652680126506No Hit
GGACAAGCAAGGTCAC11517390.9959428097586397No Hit
TTGTTGTAGCTTGTTG11422640.9877494967576358No Hit
CTACTAGTCAGGTCGA11304120.9775007214871454No Hit
TTAGGCCTCAGAAAGG11045810.9551638910777597No Hit
TTTCAAGGTCCAGCAC9496980.8212319757706915No Hit
TGACGGATCCAGGTGA9038230.7815625051721639No Hit
ACCTGGTTCCGTCCAG8342310.7213842425477992No Hit
GCGATAAGTAGCAGGT7007700.6059765648246364No Hit
TAGAGGCGTTATTGTG6421760.5553085983886663No Hit
CAACCGTTCAGCTACC6420560.555204830836147No Hit
GCACAGCAGCACAGCA6228030.5385561917640282No Hit
TTACCGGGTTACTTCT5628690.48672948765825913No Hit
TAATGCAAGGACGAAA5295180.45788989062433016No Hit
TTTGCTCTCCTAACCA4992340.43170241928687375No Hit
ATGCTTAGTTCACCTC4820970.41688354805751204No Hit
TAGGATATCAAGCTTT4125360.35673209205088147No Hit
CCTTACCGTAGTCATC3740500.3234521084987303No Hit
GGTCATGAGAAGCAAG3578410.3094357063421846No Hit
TGATGGCCAGGTTGCG3506260.3031966822469611No Hit
CGTAGTTGTTACGCCA3440920.2975465390122847No Hit
GTGGCGTAGTGAGGCC3185080.27542329681516803No Hit
GATTACTTCATAGCCC3176780.2747055712435761No Hit
TGTCAATTCATTAAGG2964550.25635341485093194No Hit
ATTTACGGTTAACGGA2576530.22280017674381328No Hit
GTGTTGCTCCAACCTA2463630.21303737951095494No Hit
TCACCTGCAGAACCGT2426520.2098283679492953No Hit
TCACCAGGTTATGGGA2407710.2082018115635551No Hit
GGCCTTAGTTATCCGG2218120.1918074029951086No Hit
GGTTTACGTATTGCGT2005970.17346216443929904No Hit
GAAGCTAGTGCTCCTT1950660.1686793449977632No Hit
CTTTGTGAGGTTGCGA1889400.16338201144165246No Hit
TCCTTGCGTACGTAAG1867440.16148306523054912No Hit
GACGAGGAGGCATGGC1738530.1503358359011623No Hit
TAGTTTGGTGGTTAAG1567910.13558181939212519No Hit
CTGAGTCTCGGTTACC1530950.13238577877453048No Hit
GCCTTCCTCCCACCCT1527640.13209955327549802No Hit
GTATCCGGTGTTAACC1523310.13172512535682418No Hit
ATTTGTGGTCGATTAC1512660.1308041883282153No Hit
TGTTTGTAGCAATACA1488220.1286907891752387No Hit
ACCTGGAAGCGCTACT1478090.12781481808605488No Hit
GTAATAGTCCGTCCCT1466960.1268523740364383No Hit
CTAGTAAAGCAGGTTG1443520.12482544784389446No Hit
GCTTAGCTCCAAATAG1374860.1188882143805813No Hit
CCCTTTAGTCTATCTT1354270.11710773612527083No Hit
CGGAACGGTCGAGGCA1342050.11605103654878252No Hit
GGTGAGGAGTTAGGAC1323140.11441583286699906No Hit
TGTTGACGTAACCGGG1314870.11370070148422017No Hit
CCTCCAACAGAGAGCA1312700.11351305516008109No Hit
CTGTGCAGTACTTATG1299510.11237247681197302No Hit
TATTGTGGTAGCTAAG1287250.1113123183170674No Hit
TTCAGGACAAATATGC1283890.11102176917001333No Hit
TCTAACCCAATGACCA1268800.10971689219708301No Hit
TCTCAATAGCTTGGTC1256940.10869132288635051No Hit
TTGAGCGAGGAGCAAA1205250.10422153556158126No Hit
CTACAACGTCACCATG1178720.1019274079213002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)