FastQCFastQC Report
Thu 12 Jan 2023
EGAF00007834587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50269980
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACTTCATCTCGTATGCCGT816730.1624687338248394No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGAAGTATCTCGTATGCCGT774370.1540422335556927Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTCTCAGATCTCGTATGCCGT714300.14209275595494567No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACTTCATCGCGTATGCCGT562540.11190376443356452No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATGGCAATCTCGTATGCCGT529400.10531136077635202No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGAAGTATCGCGTATGCCGT516680.10278102358505016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)