FastQCFastQC Report
Thu 12 Jan 2023
EGAF00007834593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007834593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50477144
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACTTCATCTCGTATGCCGT829770.1643852908952218No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGAAGTATCTCGTATGCCGT787420.15599535504623635Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTCTCAGATCTCGTATGCCGT727500.14412463589461402No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACTTCATCGCGTATGCCGT563240.11158317514952905No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGAAGTATCGCGTATGCCGT526350.10427491698024755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)