FastQCFastQC Report
Sun 21 May 2023
EGAF00007837057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44784
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG17033.8026973919256877No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15403.4387281171847093No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10832.418274383708467No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10612.369149696320114No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10602.3669167559842803No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9462.1123615576991783No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9062.0230439442658095No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8841.9739192568774562No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8031.793051089674884No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7451.6635405501964986No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7411.6546087888531618No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6311.408985351911397No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5791.2928724544480172No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5111.1410325116112898No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5081.134333690603787No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5011.1187031082529475No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4901.094140764558771No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4871.0874419435512683No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4751.0606466595212576No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4671.042783136834584No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4561.0182207931404073No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4481.0003572704537336No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4350.9713290460878885No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4230.9445337620578778No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4060.9065737763486961No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.857449088960343No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3800.8485173276170062No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3780.8440514469453377No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3750.837352625937835No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3530.7882279385494819No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3440.7681314755269739No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.739103251161129No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3290.7346373704894605No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3020.6743479814219364No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3020.6743479814219364No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.6631832797427653No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.6631832797427653No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2960.660950339406931No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2780.620757413361915No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2780.620757413361915No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2640.5894962486602359No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2590.5783315469810647No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2540.5671668453018935No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2530.5649339049660593No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2520.5627009646302251No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2470.551536262951054No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2430.5426045016077171No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2350.5247409789210432No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2270.5068774562343694No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.502411575562701No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.49571275455519825No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2210.49347981421936404No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2200.49124687388352983No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2160.482315112540193No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2140.47784923186852446No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2120.47338335119685604No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2090.4666845301893534No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2040.45551982851018225No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.4443551268310111No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1980.44212218649517687No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1910.4264916041443373No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1900.4242586638085031No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.41979278313683455No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.4153269024651661No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.4063951411218293No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1750.3907645587709897No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1650.3684351554126474No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1530.3416398713826367No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1470.3282422293676313No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1460.3260092890317971No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.32377634869596283No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.32377634869596283No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.3126116470167917No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.29251518399428367No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1300.29028224365844946No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.285816362986781No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1250.27911754197927835No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1220.27241872097177566No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1200.2679528403001072No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1140.2545551982851018No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.2433904966059307No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1070.23892461593426223No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.23892461593426223No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1040.23222579492675954No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA980.21882815291175423No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT980.21882815291175423No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA970.21659521257591996No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT960.21436227224008575No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA960.21436227224008575No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.21212933190425154No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA930.20766345123258306No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA920.20543051089674885No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG900.20096463022508038No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT890.19873168988924617No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA880.19649874955341196No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.19649874955341196No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.19426580921757772No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT870.19426580921757772No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT870.19426580921757772No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA860.19203286888174348No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG850.18979992854590927No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA850.18979992854590927No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT830.18533404787424082No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC830.18533404787424082No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.18310110753840658No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.17416934619506969No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT760.1697034655234012No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT750.167470525187567No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.16300464451589855No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT700.15630582350839586No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.15630582350839586No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC690.15407288317256163No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.15183994283672742No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC680.15183994283672742No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA680.15183994283672742No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.14960700250089318No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.14737406216505897No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.14737406216505897No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.14514112182922473No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT650.14514112182922473No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.14514112182922473No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA630.14067524115755628No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTG630.14067524115755628No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.13844230082172204No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.13620936048588783No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC610.13620936048588783No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.13620936048588783No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.1339764201500536No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA590.13174347981421936No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.12951053947838514No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGGGGCGAGCGTTG570.1272775991425509No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT560.1250446588067167No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC540.12057877813504825No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.12057877813504825No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT540.12057877813504825No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.11834583779921401No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT520.11611289746337977No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT520.11611289746337977No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA520.11611289746337977No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.11387995712754556No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.11164701679171132No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA500.11164701679171132No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.11164701679171132No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT500.11164701679171132No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT490.10941407645587711No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA490.10941407645587711No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA480.10718113612004287No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA480.10718113612004287No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG480.10718113612004287No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC470.10494819578420864No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT470.10494819578420864No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.10271525544837443No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.10048231511254019No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.10048231511254019No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA450.10048231511254019No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA450.10048231511254019No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT450.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)