FastQCFastQC Report
Sun 21 May 2023
EGAF00007837062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40678
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG40619.983283347263876No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG26966.627661143615714No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG21835.366537194552338No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG14933.670288608092827No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10972.6967894193421507No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10682.6254978120851566No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9172.2542897880918433No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8632.121539898716751No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8382.060081616598653No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7341.8044151629873642No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7181.7650818624317812No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6091.497123752396873No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5881.4454987954176706No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5681.3963321697231919No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5651.38895717586902No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4961.219332317223069No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4931.2119573233688972No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4681.150499041250799No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4271.0497074585771178No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4181.0275824770146025No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3990.9808741826048479No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3860.9489158759034367No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3760.9243325630561974No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3710.9120409066325778No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3700.9095825753478538No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3570.8776242686464428No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.8137076552436207No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3310.8137076552436207No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3200.7866660111116573No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3130.7694576921185899No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3120.766999360833866No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2770.6809577658685284No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.6760411032990805No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2730.6711244407296327No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2540.6244161463198781No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.6219578150351541No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.5850828457642951No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2220.5457495452087123No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.5236245636461969No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.5064162446531294No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2060.5064162446531294No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.4990412507989576No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.49166625694478594No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.4769162692364423No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1900.4670829440975466No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.4547912876739269No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1820.4474162938197551No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1780.4375829686808595No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.41299965583362014No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1590.3908746742711047No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1560.383499680416933No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1550.38104134913220905No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.36874969270858937No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.35645803628496975No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.3417080485766262No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1300.3195830670141108No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.30483307930576725No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.3023747480210433No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.29745808545159547No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1190.2925414228821476No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1170.28762476031269973No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1160.2851664290279758No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.28024976645852795No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1110.27287477260435616No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1090.2679581100349083No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.2532081223265647No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.24337479718766902No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA950.2335414720487733No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT920.2261664781946015No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT920.2261664781946015No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA910.22370814690987756No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.2212498156251536No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.21879148434042975No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA870.21387482177098188No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT870.21387482177098188No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.21387482177098188No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT810.19912483406263828No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC760.18683317763901863No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC750.18437484635429469No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC730.17945818378484685No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.17208318993067506No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG670.16470819607650328No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.16224986479177933No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA630.15487487093760754No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC630.15487487093760754No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT630.15487487093760754No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.15487487093760754No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT620.15241653965288363No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC600.14749987708343576No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA580.1425832145139879No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA570.14012488322926397No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA550.1352082206598161No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT540.1327498893750922No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.1327498893750922No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.1327498893750922No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA530.13029155809036824No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT530.13029155809036824No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.13029155809036824No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA520.12783322680564432No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.12537489552092038No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.12045823295147254No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.12045823295147254No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.11308323909730075No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.11308323909730075No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.10324991395840503No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA410.1007915826736811No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA410.1007915826736811No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC410.1007915826736811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)