FastQCFastQC Report
Sun 21 May 2023
EGAF00007837072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36122
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13663.7816289242013177No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11433.164276618127457No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10602.9344997508443607No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8222.275621504900061No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7852.1731908532196442No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6791.8797408781352085No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6581.821604562316594No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6511.8022257903770555No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6011.6638059908089253No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5501.5226177952494324No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5261.45617629145673No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5021.3897347876640276No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5011.3869663916726649No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4931.364819223741764No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4831.337135263828138No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4651.287304135983611No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4531.2540833840872598No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4411.2208626321909086No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4271.182105088311832No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3901.079674436631416No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3821.0575272687005148No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3801.0519904767177897No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3711.027074912795526No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3631.0049277448646254No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.9274126571064726No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.9163390731410221No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3270.9052654891755716No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3130.8665079452964953No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3090.8554343613310448No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2900.8028348374951554No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2880.79729804551243No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2860.791761253529705No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2860.791761253529705No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.7779192735728918No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2550.7059409777974641No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.700404185814739No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2480.6865622058579259No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.6394994740047616No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2240.6201207020652234No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6173523060738608No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2160.5979735341343226No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2160.5979735341343226No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.5896683461602348No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2130.5896683461602348No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.5730579702120592No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2020.559215990255246No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2010.5564475942638836No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.5536791982725209No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1950.539837218315708No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1820.5038480704279941No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1790.49554288245390626No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1780.49277448646254357No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1760.48723769447981835No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.47893250650573055No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1660.45955373456619236No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.4429433586180167No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1540.4263329826698411No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.4263329826698411No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1530.4235645866784785No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1520.4207961906871159No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1450.4014174187475777No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.3958806267648525No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.3931122307734899No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.38480704279940203No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.3820386468080394No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1290.357123082885776No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1270.35158629090305077No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.35158629090305077No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1250.34604949892032555No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.3405127069376004No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1150.3183655390066995No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1140.3155971430153369No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1140.3155971430153369No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1120.3100603510326117No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.3100603510326117No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.28237639111898566No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1020.28237639111898566No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC980.2713028071535352No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA960.26576601517081No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.2629976191794474No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA950.2629976191794474No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA950.2629976191794474No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC940.26022922318808483No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.26022922318808483No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT940.26022922318808483No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT900.2491556392226344No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC900.2491556392226344No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.24638724323127179No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT880.24361884723990918No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC860.23808205525718398No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA860.23808205525718398No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC780.21593488732628316No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT780.21593488732628316No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT770.21316649133492055No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.21039809534355794No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.21039809534355794No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC740.20486130336083275No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.20209290736947014No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.20209290736947014No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC720.1993245113781075No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19655611538674495No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA700.19378771939538234No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT690.1910193234040197No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC690.1910193234040197No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.1827141354299319No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC660.1827141354299319No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA640.1771773434472067No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC610.16887215547311887No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT610.16887215547311887No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.16333536349039365No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT590.16333536349039365No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA570.15779857150766846No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.15779857150766846No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.15503017551630585No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC560.15503017551630585No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC560.15503017551630585No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.15503017551630585No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA550.15226177952494324No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC550.15226177952494324No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA530.14672498754221805No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.1439565915508554No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.14118819555949283No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT500.13841979956813022No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.1356514035767676No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA470.13011461159404242No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT460.1273462156026798No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.1273462156026798No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.1273462156026798No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC460.1273462156026798No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.1273462156026798No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.1273462156026798No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.1245778196113172No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA450.1245778196113172No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA440.12180942361995459No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT430.11904102762859199No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.11904102762859199No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA420.11627263163722938No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA410.11350423564586679No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA400.11073583965450418No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA400.11073583965450418No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA400.11073583965450418No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.10796744366314158No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC380.10519904767177897No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC370.10243065168041637No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT370.10243065168041637No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC370.10243065168041637No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC370.10243065168041637No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA370.10243065168041637No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.10243065168041637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)