FastQCFastQC Report
Sun 21 May 2023
EGAF00007837073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36122
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13123.632135540667737No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10172.8154587232157686No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8922.4694092242954433No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8082.236863961020984No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7071.9572559658933613No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6161.7053319306793644No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6061.6776479707657383No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5951.6471956148607498No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5681.5724489230939596No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5661.5669121311112342No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5401.4949338353358064No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5241.4506394994740048No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4991.3814295996899397No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4961.3731244117158519No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4611.2762305520181605No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4471.2374730081390843No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4421.223631028182271No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4411.2208626321909086No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4331.1987154642600077No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4111.1378107524500305No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4111.1378107524500305No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3921.0852112286141409No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3701.0243065168041636No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3450.9550966170200986No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.9329494490891977No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3330.9218758651237472No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3170.8775815292619455No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2980.8249820054260562No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2930.8111400254692431No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2920.8083716294778805No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2820.7806876695642544No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.7668456896074415No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2680.741930125685178No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.7363933337024527No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2630.728088145728365No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2600.7197829577542771No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2530.700404185814739No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2510.6948673938320138No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2420.6699518299097502No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.6588782459442999No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.5702895742206966No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2060.5702895742206966No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.5509108022811583No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.5481424062897957No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1980.5481424062897957No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1960.5426056143070704No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.5370688223243453No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1900.5259952383588948No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1880.5204584463761697No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.5176900503848071No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1850.5121532584020818No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1800.4983112784452688No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1760.48723769447981835No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1730.47893250650573055No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1690.46785892254028016No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.4567853385748297No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1630.45124854659210456No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.4401749626266541No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1590.4401749626266541No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1540.4263329826698411No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1520.4207961906871159No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.4180277946957533No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.4014174187475777No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1380.3820386468080394No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.3654282708598638No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1290.357123082885776No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1210.3349759149548751No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1170.3239023309894247No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1150.3183655390066995No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1140.3155971430153369No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.3155971430153369No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.3128287470239743No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.3100603510326117No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1110.3072919550412491No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1080.2989867670671613No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.2962183710757987No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.2934499750844361No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1060.2934499750844361No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.2879131831017108No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1020.28237639111898566No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.279607995127623No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.27407120314489786No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT970.26853441116217264No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.2629976191794474No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC920.2546924312053596No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA920.2546924312053596No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT890.24638724323127179No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA880.24361884723990918No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC860.23808205525718398No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.23531365926582137No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC810.22424007530037096No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA810.22424007530037096No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT800.22147167930900835No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT800.22147167930900835No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.21593488732628316No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.21593488732628316No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA770.21316649133492055No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC770.21316649133492055No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT710.19655611538674495No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA710.19655611538674495No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA710.19655611538674495No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.19378771939538234No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC700.19378771939538234No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT700.19378771939538234No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT690.1910193234040197No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.18825092741265712No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT660.1827141354299319No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA660.1827141354299319No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC650.17994573943856929No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC640.1771773434472067No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.17164055146448148No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.17164055146448148No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.1661037594817563No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA600.1661037594817563No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC600.1661037594817563No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.1661037594817563No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT590.16333536349039365No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA570.15779857150766846No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.15779857150766846No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.15503017551630585No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC560.15503017551630585No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC560.15503017551630585No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC550.15226177952494324No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.15226177952494324No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.15226177952494324No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.15226177952494324No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.14949338353358066No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA540.14949338353358066No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.14672498754221805No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA530.14672498754221805No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA530.14672498754221805No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.1439565915508554No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG510.14118819555949283No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.13841979956813022No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC490.1356514035767676No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.1356514035767676No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA490.1356514035767676No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.132883007585405No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT480.132883007585405No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT480.132883007585405No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC480.132883007585405No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT470.13011461159404242No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT460.1273462156026798No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT460.1273462156026798No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.1245778196113172No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.1245778196113172No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT440.12180942361995459No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.12180942361995459No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC430.11904102762859199No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT430.11904102762859199No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA430.11904102762859199No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC430.11904102762859199No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA420.11627263163722938No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC410.11350423564586679No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA400.11073583965450418No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.10796744366314158No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC390.10796744366314158No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT390.10796744366314158No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.10519904767177897No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.10243065168041637No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.10243065168041637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)