FastQCFastQC Report
Sun 21 May 2023
EGAF00007837086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35712
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14514.063060035842294No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13013.6430331541218637No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11743.2874103942652333No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11273.155801971326165No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7862.200940860215054No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7091.9853270609318998No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6681.8705197132616487No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6441.80331541218638No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5981.6745071684587813No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5941.663306451612903No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5231.4644937275985663No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5121.4336917562724014No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4491.2572804659498207No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4381.2264784946236558No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4281.1984767025089607No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4131.1564740143369177No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3641.0192652329749106No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3611.0108646953405018No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3480.9744623655913979No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3460.9688620071684587No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3410.9548611111111112No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.9464605734767026No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.9380600358422938No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3180.8904569892473119No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3130.8764560931899642No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3000.8400537634408601No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2900.8120519713261649No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2770.775649641577061No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.7700492831541218No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2690.7532482078853047No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.7532482078853047No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2640.739247311827957No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2330.6524417562724014No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.6356406810035843No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.6300403225806451No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.6216397849462365No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2130.5964381720430108No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.5880376344086021No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.5852374551971326No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2060.576836917562724No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.5684363799283154No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1990.5572356630824372No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1980.5544354838709677No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.5404345878136201No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1910.534834229390681No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.5292338709677419No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1850.5180331541218638No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1820.5096326164874553No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1800.5040322580645161No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.4984318996415771No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.4956317204301075No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1730.48443100358422936No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1710.4788306451612903No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1650.4620295698924731No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.456429211469534No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1610.450828853046595No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.43682795698924726No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.4340277777777778No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1540.4312275985663082No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.4256272401433692No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1510.42282706093189965No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1450.40602598566308246No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.3892249103942652No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.37522401433691754No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1290.3612231182795699No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.35562275985663083No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1270.35562275985663083No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1240.3472222222222222No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1210.3388216845878136No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1190.33322132616487454No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1170.3276209677419355No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.31642025089605735No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.31642025089605735No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1120.31362007168458783No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.31081989247311825No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.3052195340501792No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1080.3024193548387097No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.2968189964157706No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.29401881720430106No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.29121863799283154No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA970.27161738351254483No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC960.2688172043010753No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.26601702508960573No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.26601702508960573No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.26041666666666663No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC920.25761648745519716No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT900.25201612903225806No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA850.2380152329749104No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.2380152329749104No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC840.23521505376344087No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT810.22681451612903228No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA810.22681451612903228No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA810.22681451612903228No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA790.2212141577060932No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA780.21841397849462363No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT770.2156137992831541No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.21001344086021503No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT720.20161290322580644No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19881272401433692No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19881272401433692No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA700.19601254480286737No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.19601254480286737No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC670.18761200716845877No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.18761200716845877No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.18761200716845877No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.17921146953405018No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC630.17641129032258063No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.1736111111111111No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.17081093189964158No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.17081093189964158No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC570.15961021505376344No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.15961021505376344No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC560.15681003584229392No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.15681003584229392No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT540.15120967741935484No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.15120967741935484No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.15120967741935484No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC530.1484094982078853No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.14560931899641577No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.14560931899641577No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.14280913978494625No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.14280913978494625No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.14280913978494625No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.1400089605734767No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1400089605734767No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA500.1400089605734767No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1400089605734767No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA490.13720878136200715No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA480.13440860215053765No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC470.1316084229390681No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.12880824372759858No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC460.12880824372759858No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.12880824372759858No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC450.12600806451612903No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA450.12600806451612903No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG420.11760752688172044No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC420.11760752688172044No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC410.1148073476702509No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG410.1148073476702509No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC410.1148073476702509No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC410.1148073476702509No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11200716845878135No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11200716845878135No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC400.11200716845878135No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT390.10920698924731181No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA380.1064068100358423No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA380.1064068100358423No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG380.1064068100358423No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT370.10360663082437276No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA360.10080645161290322No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA360.10080645161290322No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG360.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)