FastQCFastQC Report
Sun 21 May 2023
EGAF00007837088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42110
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13333.165518879126098No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10482.4887200189978627No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10052.3866065067679885No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8452.0066492519591543No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7381.7525528378057469No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6931.6456898598907623No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6441.5293279506055568No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6291.4937069579672286No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6071.4414628354310142No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6021.4295891712182378No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5801.3773450486820233No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5651.3417240560436952No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA5211.2372358109712658No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5121.215863215388269No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5111.2134884825457135No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5051.1992400854903824No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4991.1849916884350509No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4991.1849916884350509No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4951.1754927570648301No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4821.1446212301116123No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4761.1303728330562812No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4691.1137497031583947No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4501.0686297791498456No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4501.0686297791498456No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4421.049631916409404No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4391.0425077178817381No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4241.0068867252434102No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.959392068392306No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3820.9071479458560912No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA3700.8786511517454286No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3630.8620280218475421No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3590.8525290904773213No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA3550.8430301591071004No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3540.8406554262645453No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3480.8264070292092138No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.8097838993113275No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3280.7789123723581097No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.7575397767751127No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.6957967228686772No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2860.6791735929707908No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2790.6625504630729042No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2660.6316789361196865No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2640.6269294704345761No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2630.6245547375920208No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2520.5984326763239136No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2460.5841842792685823No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.5651864165281406No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2360.5604369508430301No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2320.5509380194728093No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2280.5414390881025885No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2220.5271906910472572No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2160.5129422939919259No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2150.5105675611493707No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.5010686297791498No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2100.49869389693659466No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2080.49394443125148424No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2080.49394443125148424No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1990.47257183566848726No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.47019710282593213No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1980.47019710282593213No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1980.47019710282593213No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.46782236998337684No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1950.4630729042982664No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.4535739729280456No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.42982664450249347No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1750.4155782474471622No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.4132035146046069No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.4108287817620518No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1710.40607931607694137No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1690.40132985039183094No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.39183091902161005No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.38945618617905486No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1610.3823319876513892No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1600.379957254808834No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.379957254808834No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1510.3585846592258371No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.35146046069817144No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1460.346710995013061No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1460.346710995013061No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1450.34433626217050584No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1420.3372120636428402No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.3300878651151746No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1370.32533839943006415No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1300.30871526953217765No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1300.30871526953217765No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1240.2944668724768464No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.29209213963429115No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.29209213963429115No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2873426739491807No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.2825932082640703No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.27309427689384946No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.26834481120873904No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.26834481120873904No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1110.2635953455236286No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2612206126810734No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.2540964141534078No Hit
TGGTACAATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT990.23509855141296607No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT990.23509855141296607No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA940.22322488720019No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT940.22322488720019No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC870.20660175730230346No Hit
TGGTACAATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.20660175730230346No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC860.20422702445974825No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC830.19710282593208261No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT790.1876038945618618No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA780.1852291617193066No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1852291617193066No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC780.1852291617193066No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC770.18285442887675138No Hit
TGGTACAATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT760.18047969603419617No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT750.17810496319164093No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.17573023034908572No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT720.1709807646639753No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT720.1709807646639753No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC700.16623129897886488No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC690.16385656613630967No Hit
TGGTACAATCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT670.15910710045119925No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.15910710045119925No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.1472334362384232No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.14485870339586798No Hit
TGGTACAATCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT610.14485870339586798No Hit
TGGTACAATCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC610.14485870339586798No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.14485870339586798No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.14248397055331277No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC600.14248397055331277No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC600.14248397055331277No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC590.14010923771075753No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA580.13773450486820232No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.13773450486820232No Hit
TGGTACAATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.13773450486820232No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.13773450486820232No Hit
TGGTACAATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.1353597720256471No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1353597720256471No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.1353597720256471No Hit
TGGTACAATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1329850391830919No Hit
TGGTACAATCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT560.1329850391830919No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA550.1306103063405367No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.1306103063405367No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT540.12823557349798148No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA540.12823557349798148No Hit
TGGTACAATCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT530.12586084065542627No Hit
TGGTACAATCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.12586084065542627No Hit
TGGTACAATCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.12586084065542627No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.12586084065542627No Hit
TGGTACAATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT510.12111137497031585No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA510.12111137497031585No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.12111137497031585No Hit
TGGTACAATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT490.11636190928520543No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA490.11636190928520543No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT490.11636190928520543No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA490.11636190928520543No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.11398717644265022No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA470.111612443600095No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA470.111612443600095No Hit
TGGTACAATCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC460.10923771075753978No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC450.10686297791498457No Hit
TGGTACAATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC450.10686297791498457No Hit
TGGTACAATCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.10448824507242936No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC440.10448824507242936No Hit
TGGTACAATCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC440.10448824507242936No Hit
TGGTACAATCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC430.10211351222987412No Hit
TGGTACAATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC430.10211351222987412No Hit
TGGTACAATCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCT430.10211351222987412No Hit
TGGTACAATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.10211351222987412No Hit
TGGTACAATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.10211351222987412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)