FastQCFastQC Report
Sun 21 May 2023
EGAF00007837118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42061
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13983.3237440859703766No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13783.2761940990466227No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12542.9813841801193504No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11092.636646774922137No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10772.560566795844131No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8472.013741946220965No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8251.9614369606048356No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7661.8211644991797626No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6591.566772069137681No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6511.5477520743681794No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT6311.500202087444426No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5981.4217446090202324No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5121.2172796652480922No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5101.2125246665557168No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4921.1697296783243385No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4891.1625971802857755No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4881.1602196809395877No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4721.1221796914005848No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4611.0960271985925203No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4411.0484772116687668No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4391.0437222129763915No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4361.0365897149378285No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4351.0342122155916407No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4261.0128147214759515No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4211.0009272247450132No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3620.86065476331994No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC3530.8392572692042509No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.7322697986258054No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3050.7251373005872424No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.6989848077791779No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.622904828701172No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.6110173319702338No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2550.6062623332778584No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2460.5848648391621692No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2450.5824873398159816No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2440.5801098404697939No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2400.5705998430850431No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2360.5610898457002924No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2310.549202348969354No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2300.5468248496231664No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2300.5468248496231664No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2240.5325598535460403No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2240.5325598535460403No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2170.5159173581227265No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2160.5135398587765388No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2080.4945198640070374No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2030.482632367276099No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1990.4731223698913483No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.4707448705451606No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.4469698770832838No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1810.43032738165997No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1800.4279498823137824No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.42319488362140706No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.42319488362140706No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1760.41843988492903167No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1700.40417488885190556No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1690.40179738950571786No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1670.3970423908133425No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.3970423908133425No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.3780223960438411No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.3756448966976534No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.35900240127433963No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.3518699032357766No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1420.33760490715865055No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1400.33284990846627516No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.3280949097738998No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1360.3233399110815245No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1300.3090749150043984No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1280.30431991631202304No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.29005492023489693No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1200.28529992154252154No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.2829224221963339No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1170.27816742350395857No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.27578992415777087No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1150.2734124248115832No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.2734124248115832No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.2734124248115832No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.2710349254653955No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1130.2686574261192078No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1130.2686574261192078No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.26627992677302015No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1080.2567699293882694No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1080.2567699293882694No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.25439243004208173No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.25439243004208173No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1040.2472599320035187No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1020.24250493331114334No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1010.24012743396495564No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.23299493592639264No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA970.23061743658020495No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC970.23061743658020495No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT960.22823993723401725No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT960.22823993723401725No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA950.2258624378878296No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA950.2258624378878296No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.2234849385416419No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG930.22110743919545423No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG920.21872993984926653No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT910.21635244050307884No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT900.2139749411568912No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT890.21159744181070353No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.21159744181070353No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.20921994246451583No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT880.20921994246451583No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA880.20921994246451583No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.20684244311832814No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA860.20446494377214047No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA850.20208744442595278No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC840.19970994507976508No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG830.19733244573357742No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.1901999476950144No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.18544494900263903No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT780.18544494900263903No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT770.18306744965645136No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT760.18068995031026366No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA760.18068995031026366No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.17831245096407597No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA730.17355745227170064No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT730.17355745227170064No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA730.17355745227170064No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.17117995292551294No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC690.1640474548869499No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA690.1640474548869499No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT670.15929245619457455No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT640.15215995815601152No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.14978245880982383No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG610.14502746011744846No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC600.14264996077126077No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.14264996077126077No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.1402724614250731No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC580.13789496207888544No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.13789496207888544No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA570.13551746273269774No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT560.13313996338651007No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.13076246404032238No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT550.13076246404032238No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1283849646941347No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT540.1283849646941347No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC540.1283849646941347No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.1283849646941347No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA520.12362996600175935No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC520.12362996600175935No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.12362996600175935No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.12125246665557167No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC510.12125246665557167No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC500.11887496730938397No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA500.11887496730938397No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT500.11887496730938397No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC500.11887496730938397No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.11887496730938397No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT490.11649746796319632No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.11649746796319632No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.11649746796319632No Hit
ATTATCAGGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT490.11649746796319632No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC490.11649746796319632No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA480.11411996861700863No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA480.11411996861700863No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.11411996861700863No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT480.11411996861700863No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC480.11411996861700863No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA470.11174246927082095No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA460.10936496992463327No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.1069874705784456No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.1069874705784456No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC450.1069874705784456No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG440.10460997123225792No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA440.10460997123225792No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC440.10460997123225792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)