FastQCFastQC Report
Sun 21 May 2023
EGAF00007837119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42061
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT15233.6209315042438366No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12893.0645966572359193No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11642.7674092389624594No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10132.4084068376881196No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9712.308551865148237No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9322.2158293906469178No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8081.9210194717196452No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7641.8164095004873875No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6971.6571170442928127No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT6171.4669170965977985No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5831.3860821188274173No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5661.3456646299422268No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5011.1911271724400274No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4931.1721071776705263No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4791.1388221868238986No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4721.1221796914005848No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4521.0746297044768311No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4381.0413447136302036No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4301.0223247188607023No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC4231.0056822234373886No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4221.003304724091201No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4180.9937947267064502No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4130.9819072299755118No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4110.9771522312831364No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.9676422338983857No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.9105822495898813No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3820.9082047502436937No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3790.9010722522051308No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3760.8939397541665676No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3290.7821972848957467No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3220.7655547894724329No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3120.7417797960105561No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.6704548156249257No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2800.6656998169325503No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2440.5801098404697939No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2410.5729773424312308No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2310.549202348969354No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2240.5325598535460403No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2200.5230498561612895No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2190.5206723568151018No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2180.5182948574689142No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2150.5111623594303512No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2140.5087848600841635No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2120.5040298613917882No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1990.4731223698913483No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.4707448705451606No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.4683673711989729No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1960.4659898718527853No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1920.4564798744680345No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1900.4517248757756592No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1900.4517248757756592No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.4469698770832838No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1830.43508238035234537No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.41130738689046864No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1720.40892988754428095No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1680.39941989015953017No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.39941989015953017No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.3756448966976534No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.37326739735146575No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.363757399966715No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1510.35900240127433963No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.3542474025819643No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1470.34949240388958897No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.3447374051972136No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.3423599058510259No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1420.33760490715865055No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1420.33760490715865055No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1390.33047240912008746No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.3209624117353368No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.3209624117353368No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1280.30431991631202304No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1250.29718741827345996No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1240.2948099189272723No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1210.28767742088870923No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1200.28529992154252154No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.2829224221963339No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1170.27816742350395857No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1160.27578992415777087No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1150.2734124248115832No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.2686574261192078No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1110.26390242742683245No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1100.26152492808064476No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1100.26152492808064476No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.2567699293882694No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1080.2567699293882694No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.25439243004208173No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1060.2520149306958941No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1050.24963743134970637No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1030.244882432657331No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.24012743396495564No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1000.23774993461876795No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC990.2353724352725803No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA980.23299493592639264No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA980.23299493592639264No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.23299493592639264No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.23299493592639264No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT970.23061743658020495No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG970.23061743658020495No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA960.22823993723401725No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA960.22823993723401725No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.2258624378878296No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG950.2258624378878296No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC910.21635244050307884No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA880.20921994246451583No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.20921994246451583No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.20921994246451583No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT880.20921994246451583No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA870.20684244311832814No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA860.20446494377214047No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT850.20208744442595278No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT850.20208744442595278No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.19733244573357742No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT830.19733244573357742No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT810.19257744704120205No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA800.1901999476950144No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT800.1901999476950144No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.18544494900263903No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC780.18544494900263903No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT760.18068995031026366No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.17831245096407597No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.17831245096407597No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.17831245096407597No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT740.1759349516178883No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.17117995292551294No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT710.16880245357932527No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG700.16642495423313758No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT690.1640474548869499No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC690.1640474548869499No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA690.1640474548869499No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC690.1640474548869499No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT690.1640474548869499No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.16166995554076224No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC670.15929245619457455No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA650.1545374575021992No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC640.15215995815601152No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA640.15215995815601152No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC620.14740495946363616No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT600.14264996077126077No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.14264996077126077No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.1402724614250731No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.13551746273269774No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.13551746273269774No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC560.13313996338651007No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC550.13076246404032238No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.12600746534794705No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT530.12600746534794705No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA530.12600746534794705No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.12600746534794705No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT520.12362996600175935No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.12125246665557167No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC500.11887496730938397No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.11411996861700863No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC480.11411996861700863No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.11411996861700863No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA470.11174246927082095No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT470.11174246927082095No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC470.11174246927082095No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.11174246927082095No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.10936496992463327No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA450.1069874705784456No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA450.1069874705784456No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.1069874705784456No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG440.10460997123225792No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA440.10460997123225792No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA430.10223247188607024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)