FastQCFastQC Report
Sun 21 May 2023
EGAF00007837122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44360
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12772.8787195671776376No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12422.799819657348963No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12062.7186654643823265No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10242.308385933273219No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9792.2069431920649234No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9782.2046889089269612No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9732.193417493237151No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9122.055906221821461No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8551.9274120829576196No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8491.9138863841298468No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8291.8688007213706042No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7691.7335437330928767No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6591.4855725879170425No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6391.4404869251577999No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6181.393146979260595No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5791.3052299368800722No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5661.2759242560865645No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5351.2060414788097384No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5231.178990081154193No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5201.1722272317403066No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4541.0234445446348062No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC4260.9603246167718664No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4170.9400360685302074No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4110.9265103697024346No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT3810.8588818755635709No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3700.8340847610459874No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3640.8205590622182146No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3430.7732191163210099No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3260.7348963029756538No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3250.7326420198376916No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3120.7033363390441839No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.6469792605951308No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2720.6131650135256989No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2550.5748422001803427No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2540.5725879170423805No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2380.5365193868349865No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2380.5365193868349865No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2320.5229936880072137No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2310.5207394048692516No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2180.4914337240757439No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2160.4869251577998197No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC2130.4801623083859332No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2110.47565374211000905No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.4733994589720469No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2080.4688908926961226No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2060.4643823264201984No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1930.4350766456266907No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1910.4305680793507664No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1900.4283137962128043No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1900.4283137962128043No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.4283137962128043No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1820.4102795311091073No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1810.40802524797114514No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1770.3990081154192967No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1740.39224526600541026No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1740.39224526600541026No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1660.3742110009017132No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1590.3584310189359784No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.35166816952209196No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1550.34941388638412985No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1540.3471596032461677No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1490.3358881875563571No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.3358881875563571No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1480.33363390441839497No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.33137962128043286No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1470.33137962128043286No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1450.3268710550045086No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.3268710550045086No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.3246167718665464No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1420.3201082055906222No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1390.3133453561767358No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1390.3133453561767358No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1370.30883678990081154No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1360.3065825067628494No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.3065825067628494No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.3043282236248873No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.2975653742110009No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1320.2975653742110009No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1300.29305680793507666No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.29080252479711455No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1280.2885482416591524No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.2885482416591524No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.2840396753832281No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1260.2840396753832281No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1240.2795311091073039No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1220.2750225428313796No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1220.2750225428313796No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1160.26149684400360684No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1150.2592425608656447No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.2569882777276826No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1130.25473399458972046No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.22993688007213708No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1010.22768259693417495No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.2254283137962128No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC990.22317403065825067No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.22091974752028853No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC940.21190261496844004No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT930.2096483318304779No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT930.2096483318304779No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT910.20513976555455365No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA890.20063119927862938No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.19837691614066727No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC860.193868349864743No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.19161406672678089No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG830.1871055004508566No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC830.1871055004508566No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.18034265103697023No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.17808836789900812No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT780.17583408476104598No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG780.17583408476104598No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC770.17357980162308384No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.17132551848512173No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC740.16681695220919748No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT740.16681695220919748No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA730.16456266907123535No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.16456266907123535No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA710.1600541027953111No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT700.15779981965734896No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT670.15103697024346258No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT670.15103697024346258No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC670.15103697024346258No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT660.14878268710550044No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT660.14878268710550044No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.14878268710550044No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT660.14878268710550044No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.14878268710550044No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT650.14652840396753833No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.14652840396753833No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA640.1442741208295762No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC630.14201983769161405No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA630.14201983769161405No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT630.14201983769161405No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA630.14201983769161405No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.1375112714156898No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA610.1375112714156898No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.1375112714156898No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.1352569882777277No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA600.1352569882777277No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.13074842200180342No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC580.13074842200180342No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT560.12623985572587917No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT560.12623985572587917No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.12623985572587917No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.12398557258791704No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA550.12398557258791704No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT550.12398557258791704No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA540.12173128944995493No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.12173128944995493No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA540.12173128944995493No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT540.12173128944995493No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT540.12173128944995493No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.12173128944995493No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.11722272317403065No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC520.11722272317403065No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.11496844003606854No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.11496844003606854No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT510.11496844003606854No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.11496844003606854No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT500.1127141568981064No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA490.11045987376014427No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.10820559062218214No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA470.10595130748422002No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC470.10595130748422002No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1036970243462579No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1036970243462579No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC460.1036970243462579No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA450.10144274120829576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)