FastQCFastQC Report
Sun 21 May 2023
EGAF00007837128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52466
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG15132.883772347806198No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG14962.8513704113139937No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11412.1747417375061944No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10572.0146380513094195No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10391.9803301185529678No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10301.9631761521747417No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT9271.7668585369572676No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9011.7173026340868374No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8861.6887126901231273No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG8581.6353447947242024No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG8441.6086608470247399No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7841.4943010711699005No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7271.385659284107803No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7031.3399153737658673No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6771.290359470895437No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6711.2789234933099531No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6351.2103076277970495No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5871.118819807113178No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5751.0959478519422103No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5441.0368619677505433No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5130.9777760835588764No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4940.9415621545381772No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4730.9015362329889833No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4670.8901002554034995No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4640.8843822666107575No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4540.8653223039682842No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4540.8653223039682842No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4500.8576983189112949No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4290.8176723973621012No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4150.7909884496626387No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.7814584683414021No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4020.7662104982274235No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.7643045019631761No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT3990.7604925094346815No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3870.7376205542637136No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3850.7338085617352189No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3570.680440666336294No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3530.6728166812793047No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC3370.6423207410513475No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3360.6404147447871003No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3330.6346967559943583No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3230.615636793351885No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3220.6137307970876378No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3160.6022948195021538No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC3100.5908588419166698No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.5832348568596806No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.5794228643311858No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3000.5717988792741966No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2960.5641748942172073No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2870.5470209278389814No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2760.5260549689322609No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.5184309838752716No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2670.508901002554035No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2490.4745930697975832No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2450.46696908474059395No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2440.4650630884763466No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2330.444097129569626No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2290.43647314451263675No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2240.4269431631914002No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2210.4212251743986582No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.3926352304349483No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT2030.38691724164220637No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2020.38501124537795905No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2010.38310524911371174No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1960.37357526779247513No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.3602332939427439No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1890.3602332939427439No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1810.3449853238287653No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.3411733313002706No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.337361338771776No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1750.33354934624328136No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1740.33164334997903405No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1720.3278313574505394No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1720.3278313574505394No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.31639537986505545No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1580.3011474097510769No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1560.2973354172225822No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1550.2954294209583349No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.2897114321655929No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1520.2897114321655929No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1420.27065146952311975No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG1410.26874547325887244No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.26493348073037776No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1380.26302748446613045No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1360.2592154919376358No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1360.2592154919376358No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.2573094956733885No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1350.2573094956733885No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1340.2554034994091412No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1340.2554034994091412No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1280.24396752182365722No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC1260.24015552929516257No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG1240.23634353676666794No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1230.2344375405024206No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.23062554797392598No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCC1200.22871955170967864No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC1180.22490755918118402No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1180.22490755918118402No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1160.22109556665268937No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT1150.21918957038844206No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.21918957038844206No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.2153775778599474No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1090.20775359280295808No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1090.20775359280295808No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1090.20775359280295808No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1070.20394160027446345No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1060.2020356040102161No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1050.2001296077459688No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.1982236114817215No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.19631761521747415No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1020.19441161895322684No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1000.19059962642473222No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.18678763389623756No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA970.18488163763199025No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.18297564136774291No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA940.1791636488392483No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.17535165631075364No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.17344566004650633No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.171539663782259No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT890.16963366751801165No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.16772767125376434No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT870.16582167498951703No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCC870.16582167498951703No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG840.16010368619677506No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC830.15819768993252772No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG820.1562916936682804No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG820.1562916936682804No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC810.1543856974040331No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT810.1543856974040331No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.15057370487553845No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT790.15057370487553845No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1486677086112911No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT780.1486677086112911No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.14294971981854915No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC750.14294971981854915No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.13913772729005452No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.13723173102580719No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.13723173102580719No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT710.13532573476155987No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC710.13532573476155987No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.13341973849731256No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT690.13151374223306522No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT690.13151374223306522No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA680.1296077459688179No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC680.1296077459688179No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT680.1296077459688179No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA660.12579575344032326No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCC650.12388975717607592No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT640.12198376091182861No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCC610.11626577211908665No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.11626577211908665No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT610.11626577211908665No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCC590.11245377959059201No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCC590.11245377959059201No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.11245377959059201No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.11054778332634468No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC580.11054778332634468No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA570.10864178706209736No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG530.10101780200510806No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT530.10101780200510806No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10101780200510806No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC530.10101780200510806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)