FastQCFastQC Report
Sun 21 May 2023
EGAF00007837155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49828
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20024.017821305290199No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT18943.8010757004094087No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18283.6686200529822592No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT17853.5823231917797225No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12362.480533033635707No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11642.33603596371518No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT8141.6336196516015093No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT6961.3968050092317572No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT6081.2201974793288914No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6071.218190575579995No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6051.2141767680822029No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5951.1941077305932408No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5921.188087019346552No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5721.1479489443686282No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5621.127879906879666No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5571.117845388135185No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5541.1118246768884965No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5451.0937625431484306No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5421.087741831901742No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5271.057638275668299No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5231.049610660672714No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5101.0235209119370636No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4970.9974311632014128No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4760.9552861844745926No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4750.9532792807256965No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4690.9412378582323192No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4530.9091273982499799No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4440.8910652645099141No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4190.8408926707875091No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.8228305370474432No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3840.7706510395761419No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3730.7485750983382837No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3650.732519868347114No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3650.732519868347114No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3360.6743196596291242No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.5920366059243799No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2860.5739744721843141No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2620.5258087822108052No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT2590.5197880709641165No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2580.5177811672152204No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.5157742634663242No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.5057397447218431No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2500.5017259372240507No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2480.49771212972625833No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2440.4896845147306735No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2270.45556715099943806No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.45155334350164567No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2210.44352572850606087No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.4395119210082684No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2130.4274704985148912No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2100.4214497872682026No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2090.4194428835193064No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2040.40940836477482534No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2010.4033876535281368No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1990.39937384603034437No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1960.3933531347836558No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.39134623103475963No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.3873324235369672No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.38131171229027855No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1880.37729790479248615No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.3572288673035241No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1770.35522196355462793No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1750.3512081560568355No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1740.3492012523079393No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1740.3492012523079393No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1690.33916673356345833No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.3371598298145621No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1680.3371598298145621No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1620.32511840732118485No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1600.32110459982339246No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1600.32110459982339246No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.3110700810789115No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.30705627358111903No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1530.30705627358111903No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.29902865858553423No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1480.297021754836638No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.297021754836638No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.297021754836638No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.29501485108774184No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1450.29100104358994944No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1410.28297342859436464No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1390.2789596210965722No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1360.27293890984988356No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1330.266918198603195No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1310.2629043911054026No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1280.25688367985871396No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1280.25688367985871396No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1270.2548767761098178No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1260.25286987236092157No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.25286987236092157No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT1260.25286987236092157No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1220.24484225736533674No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1210.24283535361644057No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1140.22878702737416712No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.22678012362527095No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.21875250862968612No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA1080.2167456048807899No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1070.21473870113189372No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1030.2067110861363089No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.20069037488962027No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT990.1986834711407241No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG980.1966765673918279No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT980.1966765673918279No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA980.1966765673918279No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT950.19065585614513927No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT950.19065585614513927No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.18864895239624307No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC940.18864895239624307No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT920.18463514489845068No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA920.18463514489845068No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.18463514489845068No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA910.18262824114955448No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.17460062615396965No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA860.17259372240507345No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC850.17058681865617725No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.17058681865617725No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA840.16857991490728105No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT810.16255920366059243No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA810.16255920366059243No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.16055229991169623No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT800.16055229991169623No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.15854539616280003No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG780.15653849241390383No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.15653849241390383No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.15653849241390383No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA740.148510877418319No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC730.14650397366942283No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA710.1424901661716304No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT710.1424901661716304No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT700.1404832624227342No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.13646945492494178No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.13044874367825318No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC640.12844183992935698No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC640.12844183992935698No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.12844183992935698No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA630.12643493618046078No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.12643493618046078No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA630.12643493618046078No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.12442803243156458No Hit
ATTATCAGGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.12442803243156458No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC600.12041422493377217No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC600.12041422493377217No Hit
ATTATCAGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT600.12041422493377217No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT590.11840732118487599No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.11840732118487599No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC580.11640041743597976No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA580.11640041743597976No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT580.11640041743597976No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA570.11439351368708356No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC570.11439351368708356No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC570.11439351368708356No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT560.11238660993818736No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.10837280244039495No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG540.10837280244039495No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT530.10636589869149876No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG530.10636589869149876No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.10435899494260256No Hit
ATTATCAGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT510.10235209119370633No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCT510.10235209119370633No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.10235209119370633No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC500.10034518744481014No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT500.10034518744481014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)