FastQCFastQC Report
Sun 21 May 2023
EGAF00007837156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47420
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15563.2813159004639396No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA14933.1484605651623787No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA14893.1400253057781526No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14563.070434415858288No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12662.66975959510755No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12042.5390130746520456No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11462.4167018135807674No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9942.0961619569801773No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9051.9084774356811471No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8231.7355546183045127No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5701.2020244622522143No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC5481.155630535638971No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5001.054407423028258No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4901.033319274567693No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4861.0248840151834668No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4791.0101223112610713No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4751.0016870518768453No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4430.9342049768030367No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4320.911008013496415No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4290.9046815689582455No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4250.8962463095740194No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4230.8920286798819064No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4220.8899198650358499No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4170.8793757908055674No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3860.8140025305778154No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3840.8097849008857022No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3800.8013496415014763No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3750.7908055672711936No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3560.7507380851961198No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.7212146773513286No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3200.6748207507380852No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3180.6706031210459722No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3180.6706031210459722No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3160.6663854913538592No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.657950231969633No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3020.6368620835090679No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2980.6284268241248419No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2960.6242091944327288No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2780.5862505272037115No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2750.579924082665542No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA2590.5461830451286377No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2580.5440742302825813No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC2430.5124420075917334No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA2320.48924504428511173No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2320.48924504428511173No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC2210.46604808097849015No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2190.46183045128637706No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.4470687473639814No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2080.4386334879797554No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2080.4386334879797554No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2000.42176296921130324No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1990.4196541543652467No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1940.40911008013496414No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1850.39013074652045554No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.3795866722901729No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.3521720792914382No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1660.35006326444538166No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1570.33108393083087306No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1560.3289751159848165No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1550.32686630113876003No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.3205398566005905No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1520.3205398566005905No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.3205398566005905No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1510.31843104175453396No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT1490.3142134120624209No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1480.3121045972163644No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.30577815267819486No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.30366933783213834No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1430.3015605229860818No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1430.3015605229860818No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.29101644875579924No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1380.29101644875579924No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1380.29101644875579924No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1360.2867988190636862No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1350.2846900042176297No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1350.2846900042176297No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1320.27836355967946014No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.27203711514129064No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1280.26992830029523407No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1280.26992830029523407No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1280.26992830029523407No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1270.26781948544917755No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.2636018557570645No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC1230.2593842260649515No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.25305778152678193No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1180.24884015183466893No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.24673133698861238No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1110.2340784479122733No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.22564318852804724No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.22564318852804724No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1060.2235343736819907No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1060.2235343736819907No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.22142555883593423No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.22142555883593423No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1040.2193167439898777No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1020.21509911429776468No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1020.21509911429776468No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA990.20877266975959508No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA990.20877266975959508No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.2066638549135386No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCT970.20455504006748207No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG940.19822859552931252No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA940.19822859552931252No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA930.196119780683256No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA890.18768452129902996No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT890.18768452129902996No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT880.18557570645297342No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA880.18557570645297342No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA860.1813580767608604No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT860.1813580767608604No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.17714044706874738No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.17714044706874738No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT840.17714044706874738No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC830.17503163222269083No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC820.17292281737663434No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC800.1687051876845213No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC800.1687051876845213No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT790.1665963728384648No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA790.1665963728384648No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.16237874314635176No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.15816111345423872No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC750.15816111345423872No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC740.1560522986081822No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA740.1560522986081822No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.1539434837621257No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.14972585407001265No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.14761703922395614No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.14761703922395614No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT700.14761703922395614No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA700.14761703922395614No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT690.14550822437789962No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC690.14550822437789962No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT690.14550822437789962No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA690.14550822437789962No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC680.1433994095318431No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT670.14129059468578659No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.13918177983973007No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCT660.13918177983973007No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.13707296499367355No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT640.13496415014761703No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.13496415014761703No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT620.130746520455504No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT620.130746520455504No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT620.130746520455504No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.130746520455504No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT610.1286377056094475No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.12652889076339097No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA600.12652889076339097No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.12442007591733446No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT590.12442007591733446No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT590.12442007591733446No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC580.12231126107127793No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT580.12231126107127793No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACC570.12020244622522143No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCT560.11809363137916491No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.11387600168705188No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA520.10965837199493884No Hit
ATTATCAGGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT520.10965837199493884No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT520.10965837199493884No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10965837199493884No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC510.10754955714888234No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC510.10754955714888234No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.10754955714888234No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC500.10544074230282581No Hit
ATTATCAGGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.10544074230282581No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10544074230282581No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA490.1033319274567693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)