FastQCFastQC Report
Sun 21 May 2023
EGAF00007837203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59723
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT30045.029887982854177No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT17182.8766136999146057No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15142.535036753009728No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG14962.5048976106357683No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13452.252063694054217No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT12712.1281583309612713No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12692.1248095373641647No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11241.8820220015739328No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11071.8535572559985267No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10351.7330006865026875No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9151.5320730706762888No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9101.523701086683522No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8261.3830517556050435No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8111.3579358036267435No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7871.3177502804614638No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7691.287611138087504No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7541.2624951861092042No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT7451.2474256149222243No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7251.2139376789511578No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6691.1201714582321718No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6141.0280796343117393No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5850.9795221271536928No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5730.959429365571053No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5420.9075230648159001No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5000.8371983992766606No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4900.8204544312911275No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4530.7585017497446546No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4490.7518041625504411No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4250.7116186393851615No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4110.688177084205415No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4020.6731075130184351No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3810.6379451802488154No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3670.6145036250690689No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3630.6078060378748555No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3590.6011084506806423No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3580.5994340538820889No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3530.5910620698893224No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3330.5575741339182559No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3220.5391557691341694No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3200.5358069755370628No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3170.5307837851414028No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2960.49562145237178307No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2940.4922726587746764No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2920.48892386517756975No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2900.48557507158046315No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.4772030875876966No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2780.4654823099978233No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2680.4487383420122901No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.43869196122097015No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2590.4336687708253102No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2510.42027359643688367No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2440.4085528188470104No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2440.4085528188470104No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2400.401855231652797No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2340.3918088508614772No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2270.3800880732716039No Hit
ATTATCAGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2220.3717160892788373No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2140.35832091489041074No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2140.35832091489041074No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2130.3566465180918574No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.3399025501063242No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2020.3382281533077709No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.3315305661135576No Hit
ATTATCAGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1910.31980978852368436No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1900.318135391725131No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1890.3164609949265777No Hit
ATTATCAGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.3164609949265777No Hit
ATTATCAGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.3147865981280244No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1870.31311220132947104No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.31143780453091774No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.31143780453091774No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1810.3030658205381511No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1760.2946938365453845No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.28967064614972454No Hit
ATTATCAGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.28967064614972454No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.28799624935117124No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1690.2829730589555113No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1610.2695778845670847No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.2679034877685314No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1550.2595315037757648No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.2578571069772115No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1540.2578571069772115No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1480.24781072618589153No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1470.2461363293873382No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1460.24446193258878487No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1450.24278753579023157No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1450.24278753579023157No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1420.2377643453945716No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1420.2377643453945716No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1410.23608994859601828No Hit
ATTATCAGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1400.23441555179746498No Hit
ATTATCAGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.23106675820035832No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.2277179646032517No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1360.2277179646032517No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1340.22436917100614506No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1310.21934598061048508No Hit
ATTATCAGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.21599718701337842No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.21599718701337842No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1280.21432279021482512No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1270.21264839341627179No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1240.20762520302061183No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1220.2042764094235052No Hit
ATTATCAGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1210.20260201262495187No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1180.19757882222929188No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.1959044254307386No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.1959044254307386No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1140.19088123503507862No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.18753244143797196No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.18753244143797196No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1120.18753244143797196No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1110.18585804463941866No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1090.18250925104231203No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1080.18083485424375867No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.17916045744520537No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1060.17748606064665204No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.17748606064665204No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1060.17748606064665204No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT1040.1741372670495454No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1000.16743967985533212No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC970.16241648945967216No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG970.16241648945967216No Hit
ATTATCAGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT960.16074209266111883No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC940.1573932990640122No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.1573932990640122No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.1506957118697989No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.1506957118697989No Hit
ATTATCAGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT890.14902131507124558No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT870.14567252147413895No Hit
ATTATCAGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.14567252147413895No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.14399812467558562No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT850.1423237278770323No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT840.140649331078479No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA830.13897493427992566No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA790.13227734708571237No Hit
ATTATCAGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC780.13060295028715904No Hit
ATTATCAGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT770.12892855348860574No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.12892855348860574No Hit
ATTATCAGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.12390536309294577No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.12223096629439244No Hit
ATTATCAGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC730.12223096629439244No Hit
ATTATCAGGCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT730.12223096629439244No Hit
ATTATCAGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC690.11553337910017916No Hit
ATTATCAGGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.11553337910017916No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.11553337910017916No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA680.11385898230162585No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA680.11385898230162585No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA660.11051018870451919No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.11051018870451919No Hit
ATTATCAGGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC650.10883579190596587No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG650.10883579190596587No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA640.10716139510741256No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.10548699830885923No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA630.10548699830885923No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA620.10381260151030591No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.10381260151030591No Hit
ATTATCAGGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC620.10381260151030591No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.10381260151030591No Hit
ATTATCAGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.1021382047117526No Hit
ATTATCAGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT600.10046380791319925No Hit
ATTATCAGGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT600.10046380791319925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)