FastQCFastQC Report
Sun 21 May 2023
EGAF00007837222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48425
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA25105.18327310273619No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT16793.467217346411977No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA16163.3371192565823438No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12852.6535880227155397No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10382.14352090862158No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT9051.8688693856479093No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9031.8647392875580795No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7991.6499741868869384No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7481.5446566855962829No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6821.408363448631905No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6791.4021683014971607No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6511.3443469282395457No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6351.3113061435209086No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5901.2183789364997417No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5581.1522973670624679No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5331.1006711409395973No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4911.013939081053175No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4780.9870934434692825No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4710.9726381001548786No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4500.9292720702116675No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4190.8652555498193082No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4180.8631905007743934No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4150.856995353639649No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4000.8260196179659267No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.7929788332472897No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3680.7599380485286525No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3580.7392875580795044No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3550.73309241094476No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3500.7227671657201858No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3350.6917914300464636No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3290.6794011357769747No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.6546205472379969No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2970.6133195663397006No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.5844088797108932No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.5761486835312339No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2670.551368094992256No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2630.5431078988125968No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.5307176045431079No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.5307176045431079No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2540.5245224574083635No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2530.5224574083634487No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA2490.5141972121837893No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2480.5121321631388746No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2410.4976768198244708No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.485286525554982No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2210.45637583892617445No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.45431078988125967No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2140.44192049561177077No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2130.439855446566856No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.4357253484770263No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.43366029943211154No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2020.41713990707279297No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2000.41300980898296336No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.4088797108931337No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1850.38203407330924105No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.36757872999483737No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1750.3613835828600929No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1750.3613835828600929No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1750.3613835828600929No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.3572534847702633No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1690.348993288590604No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.34692823954568924No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1670.3448631905007744No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3448631905007744No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1630.3366029943211151No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1620.3345379452762003No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1610.33247289623128545No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1560.3221476510067114No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.3200826019617966No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.30356220960247804No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1430.29530201342281875No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1410.29117191533298914No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1410.29117191533298914No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1380.2849767681982447No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1380.2849767681982447No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1380.2849767681982447No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.2829117191533299No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1310.270521424883841No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1260.26019617965926695No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.26019617965926695No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1210.24987093443469283No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1200.24780588538977802No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.24574083634486318No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1190.24574083634486318No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1180.24367578729994838No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.24161073825503354No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1160.23954568921011873No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1110.22922044398554467No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1100.22715539494062983No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1090.22509034589571503No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1080.2230252968508002No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1040.2147651006711409No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1020.21063500258131132No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.20856995353639649No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT990.20443985544656684No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT970.2003097573567372No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.2003097573567372No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.2003097573567372No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA970.2003097573567372No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA960.19824470831182242No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.19411461022199278No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT940.19411461022199278No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT930.19204956117707797No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.18998451213216314No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT870.17965926690758904No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT860.17759421786267424No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC850.17552916881775943No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA850.17552916881775943No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT850.17552916881775943No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA840.17346411977284462No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT830.1713990707279298No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT830.1713990707279298No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCAGGTAGTTAGACCAGCAC830.1713990707279298No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC810.16726897263810014No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT800.16520392359318534No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA780.1610738255033557No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA780.1610738255033557No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA770.1590087764584409No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC750.15487867836861124No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.15487867836861124No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA740.15281362932369644No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT730.15074858027878163No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.15074858027878163No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC730.15074858027878163No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.146618482188952No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC690.14248838409912234No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.14042333505420754No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT640.13216313887454825No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC630.13009808982963347No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT630.13009808982963347No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC620.12803304078471864No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA610.12596799173980383No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12390294269488901No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT600.12390294269488901No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC590.12183789364997419No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT580.11977284460505937No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.11977284460505937No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT570.11770779556014455No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11564274651522974No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT560.11564274651522974No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT560.11564274651522974No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.11357769747031492No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC540.1115126484254001No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT540.1115126484254001No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT540.1115126484254001No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCAGGTAGTTAGACCAGCAC530.10944759938048527No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT520.10738255033557045No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG520.10738255033557045No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC520.10738255033557045No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT510.10531750129065566No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC490.10118740320082603No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.10118740320082603No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.10118740320082603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)