FastQCFastQC Report
Sun 21 May 2023
EGAF00007837263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51018
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT16623.257673762201576No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12822.5128386059822025No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11442.2423458387235877No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10882.1325806578070488No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10802.1168999176761143No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10232.0051746442432083No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10191.9973342741777411No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9831.9267709435885374No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9401.842486965384766No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT8701.705280489239092No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8521.66999882394449No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8151.5974754008389196No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7411.4524285546277784No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7351.440667999529578No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6571.2877807832529695No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6441.2622995805402015No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5681.1133325492963269No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5511.0800109765180916No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5321.0427692187071231No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4370.8565604296522795No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.8447998745540789No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.8330393194558783No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4160.8153984868085773No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4080.7997177466776431No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3920.7683562664157748No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3890.7624759888666746No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.7526755262848407No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3830.7507154337684739No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3800.7448351562193736No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.7056333058920381No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3530.6919126582774707No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3470.6801521031792701No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3340.654670900466502No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3310.6487906229174017No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3290.6448704378846681No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3190.6252695127210005No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3090.6056685875573328No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3090.6056685875573328No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.603708495040966No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.5821474773609314No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2930.5743071072954644No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.5645066447136305No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2880.5645066447136305No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.5586263671645302No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.5449057195499628No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2770.5429456270335961No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2700.5292249794190286No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2670.5233447018699283No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2620.5135442392880945No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2550.49982359167352697No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2520.4939433141244266No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2470.4841428515425928No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2380.4665020188952918No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2290.4488611862479909No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2240.439060723666157No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.43710063114979025No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2130.4174997059861225No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.41357952095338896No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.4116194284370222No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2090.4096593359206554No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2000.39201850327335447No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1940.3802579481751539No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.3724175781096868No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.37045748559332004No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.3645772080442197No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1860.3645772080442197No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.3645772080442197No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.35281665294601905No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1770.34693637539691874No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1740.34105609784781843No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1720.33713591281508487No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1720.33713591281508487No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1720.33713591281508487No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1710.3351758202987181No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.3234152652005175No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1630.31949508016778394No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1570.3077345250695833No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1490.29205378493864914No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1480.29009369242228233No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.284213414873182No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.284213414873182No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1380.27049276725861465No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.27049276725861465No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1370.26853267474224785No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1370.26853267474224785No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.26265239719314754No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1320.258732212160414No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1280.2508918420949469No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.2508918420949469No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2469716570622133No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1250.24501156454584655No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1250.24501156454584655No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1240.24305147202947977No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.241091379513113No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1210.23717119448037946No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1180.23129091693127912No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC1180.23129091693127912No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1160.2273707318985456No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.2254106393821788No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.22149045434944528No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1120.2195303618330785No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.21757026931671172No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1050.2058097142185111No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1030.20188952918577754No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1000.19600925163667723No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.19600925163667723No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC990.19404915912031048No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC980.1920890666039437No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.19012897408757695No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC960.18816888157121017No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA940.18424869653847661No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT940.18424869653847661No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT920.18032851150574308No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.18032851150574308No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT920.18032851150574308No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG890.17444823395664277No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA880.172488141440276No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.16856795640754244No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC840.1646477713748089No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.16268767885844213No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.16268767885844213No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.16072758634207535No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC810.1587674938257086No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA810.1587674938257086No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT810.1587674938257086No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.15680740130934182No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.15288721627660826No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA780.15288721627660826No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT780.15288721627660826No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT770.1509271237602415No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC760.14896703124387473No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA750.14700693872750795No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT730.14308675369477442No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC720.14112666117840764No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC720.14112666117840764No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC710.13916656866204086No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.13916656866204086No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT710.13916656866204086No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG660.129366106080207No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT650.12740601356384024No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.12740601356384024No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA650.12740601356384024No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT650.12740601356384024No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC640.12544592104747346No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC640.12544592104747346No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.12348582853110665No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG630.12348582853110665No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC620.12152573601473989No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.11760555098200634No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG580.1136853659492728No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC570.11172527343290603No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT560.10976518091653925No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCC550.10780508840017247No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.1058449958838057No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC530.10388490336743894No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.10388490336743894No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA520.10192481085107216No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC520.10192481085107216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)