FastQCFastQC Report
Sun 21 May 2023
EGAF00007837274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50054
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA16033.2025412554441206No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA14862.968793702800975No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11462.289527310504655No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10632.1237063970911416No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9601.9179286370719622No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8091.6162544451991847No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA7671.5323450673272865No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7391.4764054820793544No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA7351.468414112758221No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7211.440444320134255No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT6171.2326687177847926No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6141.2266751907939426No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA5991.1967075558396931No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5861.1707356055460103No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA5671.1327766012706277No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5401.0788348583529788No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5291.0568585927198626No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5191.0368801694170295No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5101.01889958844448No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4890.9769448995085308No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4850.9689535301873976No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4650.9289966835817317No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4530.9050225756183323No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4500.8990290486274823No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4500.8990290486274823No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4290.8570743596915332No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4040.8071283014344508No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3990.7971390897830343No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.7791585088104847No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3870.7731649818196348No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3850.7691692971590682No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3810.761177927837935No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3680.7352059775442522No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3530.7052383425900027No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3500.6992448155991529No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3460.6912534462780198No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3300.659287968993487No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT3250.6492987573420705No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3170.6333160186998043No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3040.6073440684061214No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3020.6033483837455548No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.5953570144244217No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2900.5793742757821553No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA2790.557398010149039No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2770.5534023254884725No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA2750.5494066408279059No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.5294282175250729No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.5214368482039398No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.5054541095616735No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2500.49946058257082354No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2480.4954648979102569No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2420.4834778439285572No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.47948215926799054No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2370.47348863227714066No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2370.47348863227714066No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2360.47149078994685745No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2300.45950373596515764No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2290.4575058936348743No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2280.455508051304591No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2270.45351020897430777No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.44551883965317457No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2160.43153394334119155No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2130.4255404163503417No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2100.41954688935949175No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2070.4135533623686419No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.41155552003835855No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.40955767770807533No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2030.4055619930475087No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2030.4055619930475087No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.3855835697446758No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.38358572741439245No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT1920.38358572741439245No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1910.3815878850841092No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.3775922004235426No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1890.3775922004235426No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1870.373596515762976No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1840.3676029887721261No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1780.3556159347904263No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1630.32564829983617694No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1620.32365045750589366No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1610.32165261517561033No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1580.31565908818476046No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1460.2916849802213609No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1460.2916849802213609No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1450.28968713789107764No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.2876892955607943No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1430.28569145323051104No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1360.27170655691852796No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1360.27170655691852796No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1350.2697087145882447No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.2697087145882447No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.2697087145882447No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1350.2697087145882447No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.2657130299276781No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1320.2637151875973948No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.2617173452671115No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1300.2597195029368282No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.2597195029368282No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1290.25772166060654494No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1230.24573460662484517No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1200.23974107963399527No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1200.23974107963399527No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.237743237303712No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1170.2337475526431454No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1160.2317497103128621No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.22575618332201222No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.22176049866144565No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.21776481400087905No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1080.21576697167059578No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.21576697167059578No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC1060.21177128701002917No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.2077756023494626No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT1040.2077756023494626No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT1040.2077756023494626No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT980.19578854836776283No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA970.19379070603747953No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA930.18579933671634635No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT930.18579933671634635No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT890.17780796739521315No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA880.17581012506492988No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG880.17581012506492988No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.1718144404043633No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT860.1718144404043633No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCT850.16981659807408No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT830.1658209134135134No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACT820.1638230710832301No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC810.16182522875294683No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC800.15982738642266353No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC780.15583170176209693No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT760.15183601710153036No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC750.14983817477124706No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA740.14784033244096376No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT740.14784033244096376No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT720.14384464778039716No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCT700.13984896311983058No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG700.13984896311983058No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG690.1378511207895473No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCT670.13385543612898068No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA670.13385543612898068No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG660.1318575937986974No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT650.1298597514684141No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC640.12786190913813084No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC630.12586406680784754No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC610.12186838214728093No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT610.12186838214728093No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC600.11987053981699763No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.11987053981699763No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC590.11787269748671436No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.11787269748671436No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT590.11787269748671436No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCT570.11387701282614775No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT560.11187917049586447No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA550.10988132816558116No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA540.10788348583529789No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT540.10788348583529789No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC530.10588564350501459No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT530.10588564350501459No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCT530.10588564350501459No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.101889958844448No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.101889958844448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)