FastQCFastQC Report
Sun 21 May 2023
EGAF00007837296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57829
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA44557.7037472548375385No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA31595.462657144339345No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA30495.272441162738419No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT25944.48563869338913No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA20723.582977398882913No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT14232.460703107437445No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12382.140794411108613No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11461.9817046810423837No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11061.9125352331875012No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9111.575334174894949No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7581.3107610368500233No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT7281.2588839509588616No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7031.2156530460495598No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT6181.0686679693579346No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6071.049646371197842No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6061.0479171350014698No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5911.021978592055889No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5560.9614553251828667No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5230.9043905307025887No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT4900.8473257362223106No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4820.833491846651334No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4730.8179287208839856No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4600.7954486503311489No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.7643223987964516No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4320.747030036832731No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4280.7401130920472427No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4100.7089868405125456No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT4100.7089868405125456No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.664026699406872No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3770.6519220460322676No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3520.608691141122966No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.5965864877483615No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3410.5896695429628733No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.5792941257846409No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3310.5723771809991527No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3280.5671894724100365No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3150.5447094018571996No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3070.5308755122862232No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3030.5239585675007349No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2990.5170416227152467No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2930.5066662055370142No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2690.46516453682408476No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.4547891196458524No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2530.43749675768213175No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2520.4357675214857597No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.41155821473655085No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2370.40982897854017875No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2310.3994535613619465No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2160.3735150184163655No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2120.3665980736308772No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2110.36486883743450516No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2060.35622265645264484No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.34238876688166836No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1970.3406595306852963No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1920.332013349703436No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.332013349703436No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1830.31645022393608746No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1810.3129917515433433No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1790.3095332791505992No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1760.304345570561483No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1750.30261633436511093No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.2991578619723668No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1710.2956993895796227No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.2956993895796227No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1620.28013626381227413No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1610.2784070276159021No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1590.2749485552231579No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1580.27321931902678587No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1580.27321931902678587No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1550.26803161043766965No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1520.2628439018485535No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.26111466565218144No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1450.2507392484739491No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1440.249010012277577No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1400.24209306749208873No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1370.23690535890297254No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1320.22825917792111222No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.22307146933199604No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.2161545245465078No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.2161545245465078No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1210.20923757976101953No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1190.20577910736827543No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1170.2023206349755313No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.19713292638641514No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1130.19540369019004308No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.193674453993671No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.18848674540455482No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.18502827301181068No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.1798405644226945No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1040.1798405644226945No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1020.17638209202995037No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1020.17638209202995037No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.1746528558335783No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1010.1746528558335783No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.17292361963720623No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA990.17119438344083418No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA990.17119438344083418No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA960.166006674851718No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.16427743865534594No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG890.15390202147711357No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT890.15390202147711357No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT880.1521727852807415No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA880.1521727852807415No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA840.14525584049525325No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT840.14525584049525325No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC840.14525584049525325No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA820.14179736810250912No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA820.14179736810250912No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT800.138338895709765No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.13660965951339293No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT780.13488042331702088No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.1331511871206488No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA740.12796347853153262No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT730.12623424233516056No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA730.12623424233516056No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT700.12104653374604436No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT700.12104653374604436No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT690.11931729754967231No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC660.11412958896055611No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.11412958896055611No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT650.11240035276418406No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG640.11067111656781199No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC630.10894188037143993No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA630.10894188037143993No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT620.10721264417506786No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCAGGTAGTTAGACCAGCAC620.10721264417506786No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT600.10375417178232374No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT590.10202493558595167No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.10202493558595167No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC580.1002956993895796No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG580.1002956993895796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)