FastQCFastQC Report
Sun 21 May 2023
EGAF00007837308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84153
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG55496.59394198661961No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG53596.3681627511794No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG36754.367045738119853No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG35204.182857414471261No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT32543.8667664848549665No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT18842.2387793661545046No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT17332.0593442895678113No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14171.6838377716777775No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14031.66720140696113No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12841.5257923068696304No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12381.47112996565779No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12301.4616234715339915No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT10181.2097013772533363No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10121.2025715066604874No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9541.1336494242629498No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9521.1312728007319999No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8501.0100650006535714No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT8491.0088766888880967No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8180.9720390241583782No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT8160.9696624006274286No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8070.9589675947381556No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7630.9066818770572648No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7550.8971753829334664No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7380.876974082920395No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7170.8520195358454243No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7130.8472662887835252No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7060.8389481064252018No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6950.825876677004979No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6810.8092403122883319No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT6760.803298753460958No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6320.7510130357800672No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6050.718928618112248No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5880.6987273180991765No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5830.6927857592718025No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5590.6642662769004076No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT5560.6607013416039832No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5030.5977208180338194No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4940.5870260121445463No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4860.577519518020748No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT4730.5620714650695756No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4600.5466234121184034No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4590.5454351003529286No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4570.543058476821979No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4480.532363670932706No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4080.4848312003137143No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.43254548263282355No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3600.42779223557092444No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3580.4254156120399748No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT3500.41590911791617646No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3480.4135324943852269No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3460.41115587085427735No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3440.40877924732332777No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3260.3873896355447815No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3250.3862013237793067No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3090.3671883355317101No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3010.35768184140791176No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.333915606098416No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2800.3327272943329412No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2720.3232208002091429No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT2670.3172792413817689No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2650.31490261785081936No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2620.311337682554395No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2590.3077727472579706No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2540.30183118843059664No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2490.2958896296032227No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2370.28162988841752523No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2370.28162988841752523No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2360.28044157665205044No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.26499352370087814No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2190.260240276638979No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2160.2566753413425546No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2110.2507337825151807No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1950.23172079426758405No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1940.23053248250210923No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1920.22815585897115967No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1900.22577923544021009No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1860.22102598837831092No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.22102598837831092No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.21746105308188654No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1820.21627274131641178No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1780.2115194942545126No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1770.21033118248903782No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1760.20914287072356305No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.20795455895808823No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1690.2008246883652395No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1630.19369481777239075No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1600.1901298824759664No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1570.18656494717954203No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1570.18656494717954203No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1550.18418832364859244No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.18300001188311765No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.17824676482121848No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1500.17824676482121848No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1420.16874027069742017No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1390.1651753354009958No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1390.1651753354009958No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.16161040010457145No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1320.15685715304267225No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1300.1544805295117227No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.1532922177462479No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1250.14853897068434876No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1200.1425974118569748No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1180.14022078832602522No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1180.14022078832602522No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1150.13665585302960084No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.13665585302960084No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1140.13546754126412605No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT1130.13427922949865126No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1120.1330909177331765No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1110.13190260596770167No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.1307142942022269No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1100.1307142942022269No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1090.12952598243675212No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.12714935890580253No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1040.12358442360937816No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1030.12239611184390337No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.1200194883129538No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1000.11883117654747899No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC950.11288961772010504No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG950.11288961772010504No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT950.11288961772010504No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.10694805889273111No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC880.10457143536178153No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT870.10338312359630672No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.10100650006535715No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.10100650006535715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)