FastQCFastQC Report
Sun 21 May 2023
EGAF00007837312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64043
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG24203.7787111784269944No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG22313.4835969582936466No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT22223.4695439001920585No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA16422.563902378089721No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15752.459285167777899No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15032.3468607029651953No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA14832.3156316849616667No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT12581.9643052324219665No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11531.8003528879034398No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11151.741017753696735No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11041.723841793794794No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10511.6410848960854425No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9971.5567665474759147No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8631.347532126852271No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8011.2507221710413317No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7751.210124447636744No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7521.174211076932686No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA7161.1179988445263338No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7161.1179988445263338No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA6851.069593866620864No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6541.0211888887153944No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6370.9946442234123948No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6370.9946442234123948No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT6160.9618537545086894No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5810.907202973002514No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA5700.8900270131005731No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5410.844744936995456No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5360.836937682494574No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5210.8135159189919272No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT5200.811954468091751No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT4670.7291975703823993No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4640.72451321768187No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4580.7151445122808113No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4240.6620551816748121No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4120.6433177708726948No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT4030.629264712771107No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3970.6198960073700482No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3780.5902284402666957No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3720.5808597348656371No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3560.555876520462814No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.530893306059991No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3200.499664288056462No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3170.4949799353559327No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT3040.474681073653639No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2950.46062801555205096No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.45438221195134515No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2890.45125931015099235No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2830.44189060474993364No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2750.4293989975485221No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2730.4262760957481692No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2700.4215917430476399No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC2690.4200302921474634No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2590.404415783145699No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2520.39348562684446386No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2450.3825554705432288No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.3794325687428759No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2420.37787111784269944No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2370.37006386334181723No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.3653795106412879No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.348203550739347No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2090.32634323813687677No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2050.320097434536171No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2040.31853598363599456No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2000.3122901800352888No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1960.306044376434583No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1930.3013600237340537No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1930.3013600237340537No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.2966756710335243No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.2966756710335243No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1820.2841840638321128No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1740.27169245663070124No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.2607623003294661No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1650.2576393985291133No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1630.25451649672876037No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1620.2529550458285839No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1610.25139359492840746No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1600.249832144028231No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC1590.2482706931280546No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1510.23577908592664304No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.23577908592664304No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.2342176350264666No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1470.22953328232593728No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1400.21860312602470217No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1400.21860312602470217No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1390.2170416751245257No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1370.2139187733241728No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1360.2123573224239964No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1330.20767296972346705No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1320.2061115188232906No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1290.2014271661227613No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.19986571522258484No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1270.19830426432240839No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1260.19674281342223193No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1250.1951813625220555No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1180.1842512062208204No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1170.18268975532064394No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1150.17956685352029106No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1140.1780054026201146No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.17488250081976173No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1120.17488250081976173No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1120.17488250081976173No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1120.17488250081976173No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1110.17332104991958527No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1100.17175959901940882No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1100.17175959901940882No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.16551379541870304No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.1639523445185266No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.16239089361835016No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC1040.16239089361835016No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1020.15926799181799728No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.15770654091782083No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.15770654091782083No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC1000.1561450900176444No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1000.1561450900176444No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC950.14833783551676216No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT940.14677638461658574No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA930.14521493371640928No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT910.1420920319160564No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.1420920319160564No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC910.1420920319160564No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC910.1420920319160564No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC900.14053058101587995No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC900.14053058101587995No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT900.14053058101587995No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA880.13740767921552707No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA880.13740767921552707No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.13740767921552707No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.13584622831535062No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT870.13584622831535062No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA840.1311618756148213No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA820.1280389738144684No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.12335462111393908No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC790.12335462111393908No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.12179317021376262No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA780.12179317021376262No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT780.12179317021376262No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT770.12023171931358617No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC760.11867026841340975No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA740.11554736661305684No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA730.11398591571288041No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC720.11242446481270396No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACC710.11086301391252754No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.11086301391252754No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT710.11086301391252754No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.11086301391252754No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA710.11086301391252754No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT700.10930156301235108No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC670.10461721031182175No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT670.10461721031182175No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.10461721031182175No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA660.1030557594116453No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT660.1030557594116453No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA650.10149430851146887No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACC650.10149430851146887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)