FastQCFastQC Report
Sun 21 May 2023
EGAF00007837321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71215
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT21863.0695780383346203No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT17712.4868356385592922No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14632.0543424840272415No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG13771.9335814084111491No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13041.8310749139928386No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12821.8001825458119778No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12181.7103138383767467No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11491.6134241381731376No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT11111.5600645931334691No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10411.4617706943761848No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10051.411219546443867No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9681.359264199957874No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9481.3311802288843644No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9291.30450045636453No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8541.1991855648388683No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8521.1963771677315171No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT8521.1963771677315171No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7811.0966790704205576No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7531.0573615109176437No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7441.0447237239345644No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7191.0096187600926771No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7131.0011935687706242No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6970.9787263919118163No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6870.9646844063750614No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6550.9197500526574458No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6540.9183458541037702No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6400.8986870743523134No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6280.8818366917082074No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA6210.8720073018324791No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA6190.8691989047251282No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5750.8074141683634066No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5730.8046057712560556No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5680.7975847784876781No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5650.7933721828266518No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5440.7638840131994664No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5220.7329916450186057No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5070.7119286667134733No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4840.679632099978937No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA4820.676823702871586No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA4770.6698027101032086No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4690.6585691216738047No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4530.6361019448149968No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4520.6346977462613214No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA4410.6192515621708911No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4240.5953801867584076No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT4240.5953801867584076No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA4170.5855507968826792No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4160.5841465983290037No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4140.5813382012216527No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4070.5715088113459244No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3950.5546584287018185No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3750.5265744576283087No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3570.5012988836621498No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3560.49989468510847435No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3550.49849048655479883No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.48304430246436847No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3430.481640103910693No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3410.478831706803342No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.4690023169276135No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA3290.4619813241592361No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3160.4437267429614548No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA3050.42828055887102434No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2970.4170469704416205No Hit
ATTAAGCCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.412834374780594No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.3833462051534087No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2690.3777294109387067No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2650.37211261672400475No Hit
ATTAAGCCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2620.3679000210629783No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.36649582250930285No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2540.3566664326335744No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2510.3524538369725479No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2450.34402864565049496No Hit
ATTAAGCCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.3327950572210911No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2350.3299866601137401No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.3285824615600646No Hit
ATTAAGCCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2320.3257740644527136No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2270.31875307168433614No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.31594467457698516No Hit
ATTAAGCCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2180.30611528470125676No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.2934774977181774No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1990.2794355121814225No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1950.2738187179667205No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1910.2682019237520185No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1890.26539352664466753No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1850.25977673242996563No Hit
ATTAAGCCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1840.2583725338762901No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1800.25275573966158815No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1780.24994734255423717No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1770.24854314400056168No Hit
ATTAAGCCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.2471389454468862No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1750.2457347468932107No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.2457347468932107No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1720.24152215123218423No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1700.23871375412483328No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1660.2330969599101313No Hit
ATTAAGCCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1650.2316927613564558No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1620.22748016569542934No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1620.22748016569542934No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1610.22607596714175385No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.22045917292705186No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1540.21624657726602542No Hit
ATTAAGCCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1540.21624657726602542No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1520.21343818015867444No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1510.21203398160499895No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1480.20782138594397248No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1480.20782138594397248No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1470.206417187390297No Hit
ATTAAGCCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.206417187390297No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1470.206417187390297No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1450.20360879028294604No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1410.19799199606824405No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1400.19658779751456856No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1390.19518359896089307No Hit
ATTAAGCCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.1923752018535421No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1370.1923752018535421No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1330.18675840763884013No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1310.18395001053148916No Hit
ATTAAGCCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1300.18254581197781367No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1300.18254581197781367No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1290.18114161342413818No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1200.16850382644105877No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1190.16709962788738328No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.1656954293337078No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1170.1642912307800323No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.1628870322263568No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1150.16148283367268132No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1150.16148283367268132No Hit
ATTAAGCCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1150.16148283367268132No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1140.16007863511900583No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.15446184090430387No Hit
ATTAAGCCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.14744084813592642No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1020.14322825247489995No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA970.1362072597065225No Hit
ATTAAGCCTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT970.1362072597065225No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC960.13480306115284701No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG940.13199466404549604No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.13199466404549604No Hit
ATTAAGCCTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT920.12918626693814506No Hit
ATTAAGCCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC920.12918626693814506No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA910.12778206838446957No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC900.12637786983079408No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.12637786983079408No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.1235694727234431No Hit
ATTAAGCCTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT880.1235694727234431No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT870.12216527416976761No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA860.12076107561609212No Hit
ATTAAGCCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT840.11795267850874112No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA820.11514428140139016No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA820.11514428140139016No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA800.11233588429403918No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.11233588429403918No Hit
ATTAAGCCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.1109316857403637No Hit
ATTAAGCCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.1109316857403637No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA770.10812328863301271No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA750.10531489152566173No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA750.10531489152566173No Hit
ATTAAGCCTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.10391069297198624No Hit
ATTAAGCCTCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT740.10391069297198624No Hit
ATTAAGCCTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC740.10391069297198624No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA730.10250649441831075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)