FastQCFastQC Report
Sun 21 May 2023
EGAF00007837336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37668
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14193.767123287671233No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11543.063608367845386No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11343.010512902198152No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10602.8140596793033876No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10192.7052139747265582No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9512.524689391525964No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT7511.9937347350536265No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7341.9486035892534777No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6801.8052458320059466No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6661.768079006052883No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6451.7123287671232876No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5921.5716257831581184No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5771.531804183922693No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5761.5291494106403312No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5581.4813634915578209No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5361.4229584793458638No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4201.1150047785919084No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4111.0911118190506532No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4091.0858022724859298No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3871.0273972602739725No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.9955399808856324No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3710.9849208877561857No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3650.9689922480620154No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3630.9636827014972921No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.9610279282149305No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3580.9504088350854837No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3300.8760751831793565No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3230.8574917702028247No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3080.8176701709673995No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2980.7911224381437826No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2920.7751937984496124No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.761919932037804No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2650.7035149198258469No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2430.6451099076138898No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2350.6238717213549962No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.5521928427312308No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2080.5521928427312308No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2000.5309546564723372No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.5256451099076139No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1910.507061696931082No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1820.48316873738982696No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.46989487097801846No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.4672400976956568No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.45396623128384833No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1680.4460019114367633No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.43803759158967825No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.43538281830731657No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1600.4247637251778698No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.4247637251778698No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1550.41148985876606137No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1530.40618031220133805No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1510.40087076563661467No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1490.3955612190718913No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1460.3875968992248062No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1430.37963257937772116No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.3743230328129978No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1400.3716682595306361No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1370.363703939683551No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.363703939683551No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.35573961983646596No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.3477752999893809No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.3424657534246575No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1290.3424657534246575No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.3345014335775725No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.3318466602952108No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1250.3318466602952108No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.3318466602952108No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1230.3265371137304874No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1210.32122756716576406No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1200.31857279388340237No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.313263247318679No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.3106084740363173No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.30529892747159393No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1140.30264415418923224No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1120.29733460762450886No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1090.2893702877774238No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1080.2867155144950621No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1080.2867155144950621No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.2814059679303388No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.2760964213656154No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.2601677816714453No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.2601677816714453No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT970.2575130083890836No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT970.2575130083890836No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA960.2548582351067219No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA960.2548582351067219No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.2522034618243602No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA930.2468939152596368No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC890.23627482213019008No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.22565572900074332No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT850.22565572900074332No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA760.20176276945948818No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT730.1937984496124031No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.1911436763300414No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC710.18848890304767973No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC700.18583412976531805No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.18317935648295636No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.18317935648295636No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.18052458320059467No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.1725602633535096No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.1725602633535096No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.16990549007114794No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC630.16725071678878625No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT620.16459594350642454No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT600.15928639694170119No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC590.1566316236593395No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT580.1539768503769778No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT580.1539768503769778No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT580.1539768503769778No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.1539768503769778No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.1539768503769778No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT570.15132207709461612No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.15132207709461612No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.14866730381225443No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.14866730381225443No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC550.14601253052989274No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.14601253052989274No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA540.14335775724753105No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT540.14335775724753105No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.1407029839651694No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC530.1407029839651694No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT520.1380482106828077No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.1380482106828077No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.135393437400446No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.135393437400446No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT510.135393437400446No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT510.135393437400446No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC500.1327386641180843No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.12742911755336095No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.12742911755336095No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA480.12742911755336095No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG470.12477434427099925No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA460.12211957098863756No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.12211957098863756No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT460.12211957098863756No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC450.11946479770627588No Hit
ATTACAGACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT440.1168100244239142No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA440.1168100244239142No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC430.1141552511415525No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.11150047785919083No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC420.11150047785919083No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA420.11150047785919083No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT410.10884570457682914No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA410.10884570457682914No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.10619093129446745No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC400.10619093129446745No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.10353615801210576No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA390.10353615801210576No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG380.10088138472974409No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA380.10088138472974409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)