FastQCFastQC Report
Sun 21 May 2023
EGAF00007837337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37668
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT18544.921949665498566No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12033.1936922586811085No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10732.8485717319740895No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10532.7954762663268555No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8512.259212063289795No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8402.2300095571838168No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT8082.1450568121482423No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6241.6565785281936922No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5941.5769353297228415No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5771.531804183922693No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5661.5026016778167146No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5521.4654348518636509No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4941.311458001486673No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4741.2583625358394392No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4621.226505256451099No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4501.194647977062759No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4411.1707550175215036No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4351.1548263778273336No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4181.109695232027185No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4151.1017309121800998No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4111.0911118190506532No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3690.9796113411914622No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3610.9583731549325687No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.9026229160029733No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.8920038228735266No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.8787299564617181No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.8760751831793565No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3220.854836996920463No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2880.7645747053201656No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.7088244663905703No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2590.6875862801316768No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2540.6743124137198684No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.6716576404375066No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2530.6716576404375066No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2500.6636933205904216No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2360.626526494637358No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2130.5654667091430392No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2120.5628119358606776No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2050.5442285228841457No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.5389189763194223No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1890.5017521503663587No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.5017521503663587No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1780.47254964426038015No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1730.4592757778485717No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1730.4592757778485717No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.45131145800148664No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1700.45131145800148664No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.44334713815440163No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1660.44069236487203994No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.4327280450249549No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.4247637251778698No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1560.41414463204842306No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1490.3955612190718913No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1480.3929064457895296No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1470.3902516725071679No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1470.3902516725071679No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1450.3849421259424445No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.3822873526600828No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1410.3743230328129978No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.363703939683551No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1320.3504300732717426No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1320.3504300732717426No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1310.3477752999893809No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1310.3477752999893809No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1300.3451205267070192No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.3424657534246575No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.3345014335775725No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1230.3265371137304874No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1190.3159180206010407No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1160.3079537007539556No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1140.30264415418923224No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.2920250610597855No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.2867155144950621No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.2867155144950621No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.2840607412127004No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1070.2840607412127004No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1060.2814059679303388No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1050.2787511946479771No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.2787511946479771No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.2760964213656154No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1020.270786874800892No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.2601677816714453No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA980.2601677816714453No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT960.2548582351067219No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT950.2522034618243602No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.2522034618243602No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA900.23892959541255177No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA880.2336200488478284No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT860.228310502283105No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT860.228310502283105No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT860.228310502283105No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA850.22565572900074332No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA840.22300095571838166No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.22034618243601997No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC820.21769140915365828No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA810.2150366358712966No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC800.2123818625889349No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.20972708930657322No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT790.20972708930657322No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.20707231602421153No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT710.18848890304767973No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.18583412976531805No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.18317935648295636No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT690.18317935648295636No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.18052458320059467No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.18052458320059467No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.1752150366358713No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.1725602633535096No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT640.16990549007114794No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.16990549007114794No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA640.16990549007114794No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC640.16990549007114794No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.16194117022406285No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA610.16194117022406285No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA580.1539768503769778No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.15132207709461612No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC570.15132207709461612No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT550.14601253052989274No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC540.14335775724753105No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC540.14335775724753105No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.14335775724753105No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT530.1407029839651694No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.1407029839651694No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.135393437400446No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT510.135393437400446No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.135393437400446No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT500.1327386641180843No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.13008389083572264No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.12742911755336095No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA480.12742911755336095No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT480.12742911755336095No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA480.12742911755336095No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.12742911755336095No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA480.12742911755336095No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC470.12477434427099925No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC460.12211957098863756No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT460.12211957098863756No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.11946479770627588No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.11946479770627588No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA450.11946479770627588No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.1168100244239142No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.1168100244239142No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT430.1141552511415525No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT430.1141552511415525No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA420.11150047785919083No Hit
ATTACAGACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT410.10884570457682914No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.10884570457682914No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT410.10884570457682914No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC410.10884570457682914No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA400.10619093129446745No Hit
ATTACAGACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT400.10619093129446745No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.10619093129446745No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT400.10619093129446745No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC400.10619093129446745No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC390.10353615801210576No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG380.10088138472974409No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA380.10088138472974409No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA380.10088138472974409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)