FastQCFastQC Report
Sun 21 May 2023
EGAF00007837344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59332
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22973.8714353131531043No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20513.456819254365267No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15122.5483718735252476No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13622.2955572035326637No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13172.2197128025348882No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12862.1674644374030874No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA11241.8944245938110968No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10141.7090271691498686No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA9971.6803748398840423No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9681.6314973370188095No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9681.6314973370188095No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9621.6213847502191059No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG9241.557338367154318No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9051.525315175621924No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8291.397222409492348No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8191.3803680981595092No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7121.2000269668981327No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6911.1646329130991708No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6291.0601361828355693No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5941.001146093170633No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5760.9708083327715229No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5560.937099710105845No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5530.9320434167059934No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5510.9286725544394256No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4600.7752983213105913No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4550.7668711656441718No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4480.7550731477111845No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4400.7415896986449133No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4280.7213645250455066No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4160.7011393514461No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.6606890042472865No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.6337221061147442No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3720.6269803815816086No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3690.6219240881817568No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3640.6134969325153374No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.6101260702487696No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3610.6084406391154857No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.5966426211824984No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.5915863277826467No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3450.5814737409829435No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3320.5595631362502528No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3300.556192273983685No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3300.556192273983685No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3290.5545068428504011No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3280.5528214117171172No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3240.5460796871839817No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3230.5443942560506978No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3170.5342816692509944No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3100.5224836513180071No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3100.5224836513180071No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3060.5157419267848715No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2850.48034787298590975No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.4685498550529225No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2760.4651789927863547No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2730.4601226993865031No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.4500101125867997No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.44326838805366414No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2580.43484123238724465No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2560.43147037012067685No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2560.43147037012067685No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.4213577833209735No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2420.4078743342547024No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2320.39102002292186344No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2200.37079484932245665No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2120.3573114002561855No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.3438279511899144No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.33371536439021104No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1780.3000067417245332No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1730.2915795860581137No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1700.286523292658262No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1660.2797815681251264No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1640.2764107058585586No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1630.27472527472527475No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1610.27135441245870695No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.26966898132542305No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1600.26966898132542305No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1590.26798355019213915No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.26461268792557135No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1550.26124182565900356No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1490.2511292388593002No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.24270208319288072No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.23258949639317736No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1350.2275332029933257No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.2258477718600418No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1330.2241623407267579No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1320.222476909593474No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1310.22079147846019012No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1300.21910604732690622No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1270.21404975392705453No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.21236432279377065No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.21067889166048676No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1250.21067889166048676No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1240.20899346052720286No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.20899346052720286No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1230.20730802939391896No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.2039371671273512No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1190.2005663048607834No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1190.2005663048607834No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.1988808737274995No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.19045371806108002No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1130.19045371806108002No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.18539742466122833No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.18371199352794446No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1070.18034113126137666No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1070.18034113126137666No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.1735994067282411No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1030.1735994067282411No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG1030.1735994067282411No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.1702285444616733No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.1685431133283894No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.1685431133283894No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1000.1685431133283894No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT990.16685768219510552No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT990.16685768219510552No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT980.16517225106182162No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA970.16348681992853772No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA960.16180138879525383No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA960.16180138879525383No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC950.16011595766196993No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC930.15674509539540216No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA930.15674509539540216No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG900.15168880199555046No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT890.1500033708622666No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA880.14831793972898266No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.14663250859569876No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT870.14663250859569876No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.1449470774624149No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC850.143261646329131No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.143261646329131No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.143261646329131No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT840.14157621519584712No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG820.1382053529292793No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG800.13483449066271153No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC780.13146362839614373No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT770.12977819726285983No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.12977819726285983No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC770.12977819726285983No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC770.12977819726285983No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA770.12977819726285983No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC770.12977819726285983No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA750.12640733499629206No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC750.12640733499629206No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.12303647272972426No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT730.12303647272972426No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.12303647272972426No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.12135104159644036No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC710.11966561046315649No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.11966561046315649No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.11798017932987258No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA700.11798017932987258No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG690.11629474819658868No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.11629474819658868No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC690.11629474819658868No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC680.1146093170633048No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC660.111238454796737No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.111238454796737No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.10955302366345311No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.10618216139688533No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.10449673026360143No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.10449673026360143No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.10281129913031753No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC610.10281129913031753No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG610.10281129913031753No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.10281129913031753No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT600.10112586799703364No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.10112586799703364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)