FastQCFastQC Report
Sun 21 May 2023
EGAF00007837353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109955
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT45974.180801236869629No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA45094.100768496202992No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA44084.008912737028784No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT29192.6547223864308127No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24022.184530035014324No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG22282.026283479605293No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG21651.9689873129916786No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21571.9617116092947116No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT20361.8516665908780865No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT17201.5642762948478925No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG16291.4815151652948932No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG16121.4660542949388387No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA15681.4260379246055204No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA15541.4133054431358283No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT13981.271429221044973No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT12401.127734073029876No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG12271.1159110545223045No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT12081.0986312582420081No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11431.0395161657041516No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG11061.0058660361056797No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10920.9931335546359875No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10790.9813105361284161No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10670.9703969805829658No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT10480.9531171843026693No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT10480.9531171843026693No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10470.9522077213405484No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9920.9021872584239007No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC9480.8621708880905824No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8890.8085125733254513No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8500.7730435178027375No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8500.7730435178027375No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA8440.7675867400300124No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8430.7666772770678915No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8350.7594015733709244No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8160.742121777090628No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC8150.7412123141285071No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA8140.7403028511663862No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT7770.7066527215679141No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7660.6966486289845846No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7290.6629984993861124No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7070.6429903142194534No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6510.5920603883406849No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6350.577508980946751No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5860.5329452958028285No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT5660.5147560365604111No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5420.4929289254695103No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5380.48929107362102675No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT5300.48201536992405986No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT4990.45382201809831296No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4980.45291255513619205No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT4640.42199081442408254No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4630.4210813514619617No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC4480.4074394070301487No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT4320.3928879996362148No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4150.3774271292801601No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4140.3765176663180392No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT4130.3756082033559183No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4090.37197035150743485No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4070.3701514255831931No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3940.35832840707562186No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT3920.3565094811513801No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT3840.3492337774544132No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3800.34559592560592967No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG3780.34377699968168796No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC3670.33377290709835844No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT3570.3246782774771498No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG3560.32376881451502887No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3450.31376472193169935No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT3270.2973943886135237No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.2946659997271611No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3230.2937565367650402No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3100.281933518257469No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3040.27647674048474374No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3030.2755672775226229No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT3010.2737483515983811No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2930.26647264790141423No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2780.25283070346960124No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2780.25283070346960124No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2770.2519212405074803No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2760.25101177754535947No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2760.25101177754535947No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2730.24828338865899688No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2730.24828338865899688No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2690.24464553681051338No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2660.24191714792415078No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.24191714792415078No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC2660.24191714792415078No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2640.24009822199990905No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2620.23827929607566734No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC2580.23464144422718383No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.2300941294165795No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2510.2282752034923378No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2470.2246373516438543No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2460.22372788868173346No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2390.21736164794688737No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.20917648128779956No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2290.2082670183256787No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.20099131462871173No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCT2210.20099131462871173No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC2170.19735346278022828No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT2160.1964439998181074No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2140.19462507389386566No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC2100.1909872220453822No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.19007775908326133No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2040.185530444272657No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2040.185530444272657No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1990.18098312946205267No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC1960.17825474057569005No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.1773452776135692No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1840.16734118503023965No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1840.16734118503023965No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1830.16643172206811876No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1830.16643172206811876No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG1730.1573370924469101No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1710.15551816652266837No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.1491519257878223No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1640.1491519257878223No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1590.14460461097721794No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.14278568505297623No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.13914783320449273No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1500.13641944431813016No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1500.13641944431813016No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1500.13641944431813016No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1490.13550998135600928No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1490.13550998135600928No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG1430.13005320358328407No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1420.1291437406211632No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1410.12823427765904233No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.12732481469692147No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC1390.12641535173480062No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT1390.12641535173480062No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1370.12459642581055885No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT1370.12459642581055885No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1360.123686962848438No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.123686962848438No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT1350.12277749988631713No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1340.12186803692419627No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1330.12095857396207539No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1330.12095857396207539No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1300.11823018507571278No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1290.11732072211359192No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT1290.11732072211359192No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1280.11641125915147106No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT1270.11550179618935019No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1270.11550179618935019No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1260.11459233322722934No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT1260.11459233322722934No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT1240.11277340730298759No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC1220.11095448137874585No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT1210.11004501841662498No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1200.10913555545450412No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC1190.10822609249238324No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1180.10731662953026239No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1180.10731662953026239No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1170.10640716656814152No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1170.10640716656814152No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT1170.10640716656814152No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1150.10458824064389978No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1150.10458824064389978No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.10367877768177891No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.10185985175753717No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.10185985175753717No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1120.10185985175753717No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.10004092583329545No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1100.10004092583329545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)